COMPARISON OF RADIOACTIVE PEPTIDES OBTAINED FROM SPECIFICALLY LABELED HUMAN RENAL AND PLACENTAL ALKALINE-PHOSPHATASES

被引：12
作者：
WHITAKER, KB
MOSS, DW
机构：
来源：
BIOCHEMICAL JOURNAL | 1979年 / 183卷 / 01期
关键词：
D O I：
10.1042/bj1830189
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Peptides with different chromatographic and electrophoretic properties were obtained from human placental and renal alkaline phosphatases by tryptic digestion of the enzymes labelled with radioactive orthophosphate at their active centres. These results provide structural evidence for the distinct genetic origins of the two isoenzymes that had previously been inferred from their different properties and from the observed phenotypic variation of placental phosphatase.
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页码：189 / 192
页数：4
相关论文
共 24 条
[1]   STRUCTURAL EVIDENCE THAT HUMAN LIVER AND PLACENTAL ALKALINE-PHOSPHATASE ISOENZYMES ARE CODED BY DIFFERENT GENES [J].
BADGER, KS ;
SUSSMAN, HH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2201-2205
[2]   CATALYTIC-CENTRE ACTIVITY AND KINETIC PROPERTIES OF BOVINE MILK ALKALINE PHOSPHATASE [J].
BARMAN, TE ;
GUTFREUN.H .
BIOCHEMICAL JOURNAL, 1966, 101 (02) :460-&
[3]   .5. USE OF ANTIBODIES IN DISCRIMINATION OF MULTIPLE MOLECULAR FORMS OF ENZYMES - HUMAN ORGAN ALKALINE PHOSPHATASES - DISCRIMINATION BY SEVERAL MEANS INCLUDING STARCH GEL ELECTROPHORESIS OF ANTIENZYME-ENZYME SUPERNATANT FLUIDS [J].
BOYER, SH .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1963, 103 (02) :938-&
[4]   RARE VARIANTS OF PLACENTAL ALKALINE-PHOSPHATASE [J].
DONALD, LJ ;
ROBSON, EB .
ANNALS OF HUMAN GENETICS, 1974, 37 (JAN) :303-313
[5]  
EATON RH, 1968, ENZYMOLOGIA, V35, P31
[6]   STUDIES ON CALF-INTESTINAL ALKALINE PHOSPHATASE .2. INCORPORATION OF INORGANIC PHOSPHATE INTO A HIGHLY PURIFIED ENZYME PREPARATION [J].
ENGSTROEM, L .
BIOCHIMICA ET BIOPHYSICA ACTA, 1961, 52 (01) :49-&
[7]   AMINO ACID SEQUENCE AROUND REACTIVE SERINE IN CALF-INTESTINAL ALKALINE PHOSPHATASE [J].
ENGSTROM, L .
BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 92 (01) :79-&
[8]   HUMAN ALKALINE-PHOSPHATASES .1. PURIFICATION AND SOME STRUCTURAL-PROPERTIES OF ENZYME FROM HUMAN LIVER [J].
GERBITZ, KD ;
KOLB, HJ ;
WIELAND, OH .
HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1977, 358 (04) :435-446
[9]   PURIFICATION AND PROPERTIES OF MOLECULAR-WEIGHT VARIANTS OF HUMAN PLACENTAL ALKALINE PHOSPHATASE [J].
GHOSH, NK ;
FISHMAN, WH .
BIOCHEMICAL JOURNAL, 1968, 108 (05) :779-+
[10]   STUDIES ON HUMAN PLACENTAL ALKALINE PHOSPHATASE .I. PURIFICATION AND CRYSTALLIZATION [J].
HARKNESS, DR .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1968, 126 (02) :503-&
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