HARPIN, ELICITOR OF THE HYPERSENSITIVE RESPONSE PRODUCED BY THE PLANT PATHOGEN ERWINIA-AMYLOVORA

被引：692

作者：

WEI, ZM ^{[1
]}

LABY, RJ ^{[1
]}

ZUMOFF, CH ^{[1
]}

BAUER, DW ^{[1
]}

HE, SY ^{[1
]}

COLLMER, A ^{[1
]}

BEER, SV ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT PLANT PATHOL, ITHACA, NY 14853 USA

来源：

SCIENCE | 1992年 / 257卷 / 5066期

关键词：

D O I：

10.1126/science.1621099

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A proteinaceous elicitor of the plant defense reaction known as the hypersensitive response was isolated from Erwinia amylovora, the bacterium that causes fire blight of pear, apple, and other rosaceous plants. The elicitor, named harpin, is an acidic, heat-stable, cell-envelope-associated protein with an apparent molecular weight of 44 kilodaltons. Harpin caused tobacco leaf lamina to collapse and caused an increase in the pH of bathing solutions of suspension-cultured tobacco cells. The gene encoding harpin (hrpN) was located in the 40-kilobase hrp gene cluster of E. amylovora, sequenced, and mutated with Tn5tac1. The hrpN mutants were not pathogenic to pear, did not elicit the hypersensitive response, and did not produce harpin.

引用

页码：85 / 88

页数：4

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