PROCESS-DEVELOPMENT FOR THE PRODUCTION OF RECOMBINANT ANTIBODIES USING THE GLUTAMINE-SYNTHETASE (GS) SYSTEM

被引：71

作者：

BROWN, ME

RENNER, G

FIELD, RP

HASSELL, T

机构：

[1] Celltech Ltd., Slough, SL1 4EN, Berks

来源：

CYTOTECHNOLOGY | 1992年 / 9卷 / 1-3期

关键词：

RECOMBINANT ANTIBODIES; GLUTAMINE SYNTHETASE; PROCESS DEVELOPMENT; CELL LINE STABILITY;

D O I：

10.1007/BF02521750

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

GS technology has been successfully utilised for the expression of recombinant antibodies. The vectors are capable of stable product accumulation over the extended periods required for large scale manufacture. Large scale processes have been developed giving extremely high antibody yields up to, and in one instance in excess of 1 g/l. It is clear that such systems represent a significant advance over previous technology and will speed the commercial application of such therapeutic products. © 1992 Kluwer Academic Publishers.

引用

页码：231 / 236

页数：6

共 9 条

[1] HIGH-LEVEL EXPRESSION OF A RECOMBINANT ANTIBODY FROM MYELOMA CELLS USING A GLUTAMINE-SYNTHETASE GENE AS AN AMPLIFIABLE SELECTABLE MARKER [J].

BEBBINGTON, CR ;

RENNER, G ;

THOMSON, S ;

KING, D ;

ABRAMS, D ;

YARRANTON, GT .

BIO-TECHNOLOGY, 1992, 10 (02) :169-175

[2] HIGH-LEVEL EXPRESSION OF TISSUE INHIBITOR OF METALLOPROTEINASES IN CHINESE HAMSTER OVARY CELLS USING GLUTAMINE-SYNTHETASE GENE AMPLIFICATION [J].

COCKETT, MI ;

BEBBINGTON, CR ;

YARRANTON, GT .

BIO-TECHNOLOGY, 1990, 8 (07) :662-667

[3]

FIELD RP, 1990, PRODUCTION BIOL ANIM, P742

[4] ENVIRONMENTAL-EFFECTS ON PROTEIN GLYCOSYLATION [J].

GOOCHEE, CF ;

MONICA, T .

BIO-TECHNOLOGY, 1990, 8 (05) :421-427

[5]

Meister A., 1980, GLUTAMINE METABOLISM, P1

[6] HIGH-LEVEL EXPRESSION OF THE HUMANIZED MONOCLONAL-ANTIBODY CAMPATH-1H IN CHINESE-HAMSTER OVARY CELLS [J].

PAGE, MJ ;

SYDENHAM, MA .

BIO-TECHNOLOGY, 1991, 9 (01) :64-68

[7]

SAHAGAN BG, 1986, J IMMUNOL, V137, P1066

[8] THE FUNCTIONAL EXPRESSION OF ANTIBODY FV FRAGMENTS IN ESCHERICHIA-COLI - IMPROVED VECTORS AND A GENERALLY APPLICABLE PURIFICATION TECHNIQUE [J].

SKERRA, A ;

PFITZINGER, I ;

PLUCKTHUN, A .

BIO-TECHNOLOGY, 1991, 9 (03) :273-278

[9]

WOOD CR, 1990, J IMMUNOL, V145, P3011

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