RAT SERUM RIBONUCLEASE

被引:13
作者
UMEDA, T
MORIYAMA, T
OURA, H
TSUKADA, K
机构
[1] Department of Biochemistry, Drug Research Institute, Faculty of Pharmaceutical Science, Gofuku, Toyama
关键词
D O I
10.1016/0005-2744(69)90159-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rat serum ribonuclease has been purified about 6000-fold. The optimal pH of this enzyme was 7-8. The purified enzyme was rather heat labile at pH 7.6. The Km of this enzyme is 30% higher than that of the crystalline bovine pancreatic ribonuclease (polyribonucleotide 2-oligonucleotide-transferase, EC 2.7.7.16). No stimulatory or inhibitory effect on this enzyme was observed with EDTA, KCl, MgCl2. p-chloromercuribenzoinc acid, or mercaptoethanol. © 1969.
引用
收藏
页码:260 / &
相关论文
共 12 条
[1]  
ALEXANDROWICZ J, 1955, SANG, V26, P212
[2]  
COELHO E, 1925, BIOCHEM Z, V161, P71
[3]  
JONES W, 1920, NUCLEIC ACIDS THEIR, P91
[4]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[5]  
METAIS P, 1955, B SOC CHIM BIOL PARI, V37, P99
[6]  
MORIYAMA T, TO BE PUBLISHED
[7]   COLUMN CHROMATOGRAPHY OF PROTEINS - SUBSTITUTED CELLULOSES [J].
PETERSON, EA ;
SOBER, HA .
METHODS IN ENZYMOLOGY, 1962, 5 :3-27
[8]   CHARACTERISTICS OF RAT AND GUINEA PIG SERUM RIBONUCLEASE [J].
RABINOVITCH, M ;
DOHI, SR .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1957, 70 (01) :239-247
[9]  
ROTH JS, 1952, J BIOL CHEM, V196, P489
[10]   ISOLATION AND PHYSICAL PROPERTIES OF RIBOSOMAL RIBONUCLEIC ACID OF ESCHERICHIA COLI [J].
STANLEY, WM ;
BOCK, RM .
BIOCHEMISTRY, 1965, 4 (07) :1302-&