USE OF THE POLYMERASE CHAIN-REACTION TO AMPLIFY TOMATO YELLOW LEAF CURL VIRUS-DNA FROM INFECTED PLANTS AND VIRULIFEROUS WHITEFLIES

被引：47

作者：

NAVOT, N

ZEIDAN, M

PICHERSKY, E

ZAMIR, D

CZOSNEK, H

机构：

[1] HEBREW UNIV JERUSALEM,DEPT FIELD & VEGETABLE CROPS,POB 12,IL-76100 REHOVOT,ISRAEL

[2] HEBREW UNIV JERUSALEM,FAC AGR,OTTO WARBURG CTR BIOTECHNOL AGR,IL-76100 REHOVOT,ISRAEL

[3] UNIV MICHIGAN,DEPT BIOL,ANN ARBOR,MI 48109

来源：

PHYTOPATHOLOGY | 1992年 / 82卷 / 10期

关键词：

VIRUS TYPING;

D O I：

10.1094/Phyto-82-1199

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The genomic DNA molecule of an Israeli isolate of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected plants (Lycopersicon esculentum 'M82') by the use of the polymerase chain reaction (PCR). Synthetic oligonucleotides complementary to different regions of the viral genome were used in different combinations to amplify the full-length as well as subgenomic fragments of the TYLCV DNA molecule. The procedure was also used to amplify TYLCV DNA from nucleic acid extracts of individual whiteflies (Bemisia tabaci) fed on TYLCV-infected tomato. TYLCV DNA was also amplified from whiteflies collected from naturally infected bean plants (Phaseolus vulgaris 'Tender Green') in the field.

引用

页码：1199 / 1202

页数：4

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