DIHYDROPTERIDINE REDUCTASE-ACTIVITY IN ELUATES FROM DRIED BLOOD SPOTS - AUTOMATION OF AN ASSAY FOR A NATIONAL SCREENING SERVICE

被引：10
|
作者：
SURPLICE, IM
GRIFFITHS, PD
GREEN, A
LEEMING, RJ
机构：
[1] Clinical Chemistry Department, The Children's Hospital, Birmingham
来源：
JOURNAL OF INHERITED METABOLIC DISEASE | 1990年 / 13卷 / 02期
关键词：
D O I：
10.1007/BF01799682
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
We describe the automation, using a Cobas Bio centrifugal analyser, of a method for dihydropteridine reductase (DHPR) assay in eluates from dried blood spots. This is used as part of a routine screening service of neonates with hyperphenylalaninaemia in the United Kingdom. Automation reduced reagent volumes and analysis time by about 80% and improved the precision of the assay. Relating DHPR activity to haemoglobin concentration of the eluate further improved the precision of the assay and removed some differences in 'apparent' DHPR activity between samples from different countries and different age groups. © 1990 Society for the Study of Inborn Errors of Metabolism and Kluwer Academic Publishers.
引用
收藏
页码：169 / 177
页数：9
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