PHENYLALANINE HYDROXYLASE ACTIVITY IN MAMMALIAN CELLS

被引：100

作者：

TOURIAN, A

GODDARD, J

PUCK, TT

机构：

[1] University of Colorado Medical Center, Denver, Colorado

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1969年 / 73卷 / 02期

关键词：

D O I：

10.1002/jcp.1040730210

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Survey of twelve mouse tissues revealed the presence of appreciable phenylalanine hydroxylase activity in the pancreas and kidney as well as the liver but in no other of the tissues tested. Single cell suspensions of mouse liver were prepared by use of tetraphenylboron. The enzyme activity of such suspensions was much more stable than that of liver extracts, and permitted determination of the Michaelis‐Menten constant, the pseudo‐first order reaction velocity constant on a cell‐number basis, and the temperature coefficient and apparent activation energy of the enzyme activity. Possible applications of these methods to problems in cellular biology have been indicated. Copyright © 1969 Wiley‐Liss, Inc.

引用

页码：159 / &

共 26 条

[1]

ANDERSON JA, 1966, PHENYLKETONURIA A ED, P193

[2]

BRENNEMAN AR, 1965, J BIOL CHEM, V240, P3617

[3]

COLOWICK, 1962, METHODS ENZYMOLOG ED, V5, P809

[4]

COLOWICK SP, 1957, METHODS ENZYMOLOG ED, V3, P447

[5]