RADIORECEPTOR ASSAY FOR BENZODIAZEPINES

被引：46
作者：
HUNT, P
HUSSON, JM
RAYNAUD, JP
机构：
[1] Centre de Recherches Roussel Uclaf, Romainville
来源：
JOURNAL OF PHARMACY AND PHARMACOLOGY | 1979年 / 31卷 / 07期
关键词：
D O I：
10.1111/j.2042-7158.1979.tb13551.x
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A simple, rapid and sensitive radioreceptor assay for determining benzodiazepines in serum is based on the displacement by the drug of specific [3H]diazepam binding to a membrane fraction from rat brain. The limit of detection of the more active benzodiazepines is about 0.5 ng. Diazepam, nitrazepam, clobazam and HR 458t have been assayed in human serum after a single oral clinical dose. The results can be used for determining pharmacokinetic parameters. The technique measures not only the parent benzodiazepine but also clinically active metabolites. 1979 Royal Pharmaceutical Society of Great Britain
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页码：448 / 451
页数：4
相关论文
共 16 条
[1]   CENTRAL NERVOUS-SYSTEM ACTIVITY OF A NOVEL CLASS OF ANNELATED 1,4-BENZODIAZEPINES, AMINOMETHYLENE-2,4-DIHYDRO-1H-IMIDAZO[1,2-A][1,4]BENZODIAZEPIN-1-ONES [J].
AGER, IR ;
DANSWAN, GW ;
HARRISON, DR ;
KAY, DP ;
KENNEWELL, PD ;
TAYLOR, JB .
JOURNAL OF MEDICINAL CHEMISTRY, 1977, 20 (08) :1035-1041
[2]   PHARMACOKINETICS OF DIAZEPAM IN DISORDERED LIVER-FUNCTION [J].
ANDREASEN, PB ;
HENDEL, J ;
GREISEN, G ;
HVIDBERG, EF .
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 1976, 10 (02) :115-120
[3]   DELAYED RECOVERY FROM A SEDATIVE - CORRELATION OF PLASMA LEVELS OF DIAZEPAM WITH CLINICAL EFFECTS AFTER ORAL AND INTRAVENOUS ADMINISTRATION [J].
BAIRD, ES ;
HAILEY, DM .
BRITISH JOURNAL OF ANAESTHESIA, 1972, 44 (08) :803-&
[4]  
BARZAGHI F, 1973, ARZNEIMITTELFORSCH, V23, P683
[5]   HIGH-DENSITIES OF BENZODIAZEPINE RECEPTORS IN HUMAN CORTICAL AREAS [J].
BRAESTRUP, C ;
ALBRECHTSEN, R ;
SQUIRES, RF .
NATURE, 1977, 269 (5630) :702-704
[6]   PHARMACOLOGICAL CHARACTERIZATION OF BENZODIAZEPINE RECEPTORS IN BRAIN [J].
BRAESTRUP, C ;
SQUIRES, RF .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1978, 48 (03) :263-270
[7]   PLASMA-LEVEL PROFILE OF NITRAZEPAM (MOGADON) FOLLOWING ORAL-ADMINISTRATION [J].
BREIMER, DD ;
BRACHT, H ;
DEBOER, AG .
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY, 1977, 4 (06) :709-711
[8]   SIMPLE AND SENSITIVE RADIORECEPTOR ASSAY FOR ANTI-SCHIZOPHRENIC DRUGS IN BLOOD [J].
CREESE, I ;
SNYDER, SH .
NATURE, 1977, 270 (5633) :180-182
[9]  
GARATTINI S, 1973, BIOL EFFECTS DRUGS R, P211
[10]  
IISALO E, 1977, BRIT J CLIN PHARMACO, V4, P646
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