5BETA-CHOLESTANE-3ALPHA,7ALPHA,12ALPHA,26-TETROL-NAD+ OXIDOREDUCTASE AND 5BETA-CHOLESTANE-3ALPHA,7ALPHA,12ALPHA-TRIOL-26AL-NAD+ OXIDOREDUCTASE IN RAT LIVER

被引：7

作者：

OKUDA, K

TAKIGAWA, N

FUKUBA, R

KUWAKI, T

机构：

[1] Department of Biochemistry, Hiroshima University, School of Dentistry, Hiroshima

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 185卷 / 01期

关键词：

D O I：

10.1016/0005-2744(69)90276-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5β-Cholestane-3α,7α,12α,26-tetrol was dehydrogenated to 5β-cholestane-3α,7α,12α-triol-26-oic acid by two separate enzymes in rat liver soluble fraction, i.e. 5β-cholestane-3α,7α,12α,26-tetrol:NAD+ ixidoreductase and 5β-cholestane-3α,7α,12α-triol-26-al:NAD+ oxidoreductase. These two enzymes were separated by gel filtration on Sephadex G-100. Their assay methods and some properties are described. © 1969.

引用

页码：1 / &

共 9 条

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[2] THE ASSAY AND SPECIFIC ACTIVITY OF CRYSTALLINE ALCOHOL DEHYDROGENASE OF HORSE LIVER
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[5] SEPARATION OF 5BETA-CHOLESTANE-3ALPHA,7ALPHA,12ALPHA,26-TETROL OXIDOREDUCTASE ETHANOL-NAD OXIDOREDUCTASE 5BETA-CHOLESTANE-3ALPHA,7ALPHA,12ALPHA-TRIOL-26-AL OXIDOREDUCTASE AND ACETALDEHYDE-NAD OXIDOREDUCTASE FROM SOLUBLE FRACTION OF RAT LIVER BY GEL FILTRATION
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TAKIGAWA, N
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1968, 33 (05) : 788 - &
[6] SYNTHESIS AND METABOLISM OF 5 BETA-CHOLESTANE-3 ALPHA 7 ALPHA 12 ALPHA-TRIOL-26-AL - BILE ACIDS AND STEROIDS 161
OKUDA, K
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[J]. ACTA CHEMICA SCANDINAVICA, 1965, 19 (09): : 2160 - +
[7] OKUDA K, 1969, BIOCHIM BIOPHYS ACTA, V176, P873
[8] RACKER E, 1949, J BIOL CHEM, V177, P883
[9] LIVER ALDEHYDE DEHYDROGENASE
RACKER, E
[J]. METHODS IN ENZYMOLOGY, 1955, 1 : 514 - 517

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