THE ROLE OF CALCIUM IN NEUTROPHIL MIGRATION - THE EFFECT OF CALCIUM AND CALCIUM-ANTAGONISTS IN ELECTROPORATED NEUTROPHILS

被引：29
作者：
ELFERINK, JGR
BOONEN, GJJC
DEKOSTER, BM
机构：
[1] Department of Medical Biochemistry, University of Leiden, 2300 RA Leiden
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 02期
关键词：
D O I：
10.1016/0006-291X(92)91812-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Chemotaxis by electroporated rabbit peritoneal neutrophils in the absence of Ca2+ is only slightly different from that in the presence of Ca2+. Pretreatment of neutrophils with quin2-AM causes inhibition of chemotaxis. Calcium antagonists as nitrendipine and verapamil are inhibitory in nanomolar concentrations, while 105 times higher concentrations are required for inhibition of chemotaxis by neutrophils which were not electroporated. The results support the hypothesis that Ca2+ from Ca2+-storing organelles is of importance for chemotaxis, but that chemotaxis is not dependent on changes in cytoplasmic Ca2+ concentrations. © 1992.
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页码：864 / 869
页数：6
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