Resin Infiltration into Differentially Extended Experimental Carious Lesions

Resin infiltration of initial caries lesions is a novel method of caries therapy. However, it has some limitations. Therefore, further experimental studies are needed to improve resin infiltration. It was the aim of this investigation to study resin infiltration into different experimental carious lesions. Caries-free extracted human molars and premolars were demineralized for 3, 6, 9 and 12 days and infiltrated with resin. Prior to infiltration, the teeth were incubated with sodium fluorescein. After embedding, serial sections were cut through the experimental lesions, and the penetration of the resin was measured with fluorescence microscopy. Two infiltrated teeth from each time interval were not embedded and cut. Infiltration of the resin was then studied with EDS element analysis. The results showed that with increasing demineralization time, the lesion expansion was also increasing, and the resin infiltration was always almost complete. From these results it can be concluded that artificial standardized caries-like lesions are suitable for experimental studies of resin infiltration.