STUDIES ON THE BIOSYNTHESIS OF SULFOLIPIDS IN THE DIATOM NITZSCHIA-ALBA

被引:14
作者
ANDERSON, R
KATES, M
VOLCANI, BE
机构
[1] UNIV OTTAWA,DEPT BIOCHEM,OTTAWA K1N 6N5,ONTARIO,CANADA
[2] UNIV CALIF SAN DIEGO,SCRIPPS INST OCEANOG,LA JOLLA,CA 92093
关键词
(Nitzschia alba); Deoxyceramide sulfonate; Diatoms; Phosphatidyl sulfocholine; Sulfolipid synthesis;
D O I
10.1016/0005-2760(79)90230-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Labeling of sulfolipids in Nitzschia alba was studied after growth of the cells in media containing ll-[35S] cystine, l-[35S] cysteine, l-[35S]-methionine or a mixture of l-[Me-3H] methionine and l-[35S] methionine. [35S] Cysteine or [35S] cystine labeled the deoxyceramide sulfonate and the sulfonium analog, phosphatidylsulfocholine (and its lyso derivative) but not the sterol sulfate nor the sulfoquinovosyl diglyceride; [35S] methionine labeled only the phosphatidylsulfocholine and its lyso derivative. With the [35S] - and [Me-3H] methionine mixture ( 3H 35S ratio 1.0) the phosphatidylsulfocholine had a 3H 35S ratio of 1.5 indicating that both sulfonium methyl groups were derived from methionine. Probable biosynthetic pathways for these novel sulfolipids are discussed. © 1979.
引用
收藏
页码:557 / 561
页数:5
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