REVERSIBLE INHIBITION OF ACETYLCHOLINE SYNTHESIS AND BEHAVIORAL-EFFECTS CAUSED BY 3-BROMOPYRUVATE

被引：15

作者：

ARENDT, T

SCHUGENS, MM

MARCHBANKS, RM

机构：

[1] INST PSYCHIAT,DEPT BIOCHEM,DENMARK HILL,DECRESPIGNY PK,LONDON SE5 8AF,ENGLAND

[2] INST PSYCHIAT,DEPT PSYCHIAT,LONDON SE5 8AF,ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 55卷 / 05期

基金：

英国惠康基金;

关键词：

Acetylcholine; Basal fore‐brain; Bromopyruvate; Cholinergic system; Memory; Pyruvate decarboxylase;

D O I：

10.1111/j.1471-4159.1990.tb04928.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: 3‐Bromopyruvate inhibits pyruvate decarboxylase in brain homogenates and causes a 90% drop in acetylcholine tissue content at a concentration of 2 mM. Stereotaxic injection of 3‐bromopyruvate into the basal forebrain causes after 7 days a 40% drop of acetylcholine concentration and pyruvate decarboxylase activity in the cortex and hippocampus, and greater decreases at the site of injection. However, values return to normal 18 days after injection. Choline ace‐tyltransferase is partially inhibited only at the site of injection after 7 days. Choline transport and choline concentration are not affected at either 7 or 18 days after injection. Impairments in spontaneous alternation and in retention of passive avoidance were seen only 7 days after the injection. The results suggest that stereotaxic injection of bromopyruvate can induce discrete reversible cholinergic lesions on a time scale useful for behavioural experiments and for comparison with neurodegeneration. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：1474 / 1479

页数：6

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