CLONING AND CHARACTERIZATION OF A DEVELOPMENTALLY-REGULATED SEA-URCHIN CDNA-ENCODING GLUTAMINE-SYNTHETASE

被引：12

作者：

FUCCI, L ^{[1
]}

PISCOPO, A ^{[1
]}

ANIELLO, F ^{[1
]}

BRANNO, M ^{[1
]}

DIGREGORIO, A ^{[1
]}

CALOGERO, R ^{[1
]}

GERACI, G ^{[1
]}

机构：

[1] Staz Zool Anton Dohrn, I-80136 NAPLES, ITALY

来源：

GENE | 1995年 / 152卷 / 02期

关键词：

RECOMBINANT DNA; EMBRYO; PARACENTROTUS LIVIDUS; MESSENGER-RNA CONTROL; COMPARISON WITH HUMAN AND LOBSTER GS;

D O I：

10.1016/0378-1119(94)00719-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A 2935-bp cDNA clone encoding glutamine synthetase (GS) was isolated from a cDNA library prepared from fourblastomere Paracentrotus lividus sea urchin embryos. The sequence consists of a 75-bp 5' untranslated region (5'-UTR) followed by a 1095-bp coding region corresponding to a 365-amino-acid (aa) protein, a 1747-bp 3'-UTR and a terminal 18-bp poly(A) tail. The encoded protein shows about 66% identical residues, as compared with human and lobster class-II GS, The sequence contains the Mn2+-binding aa and the highly conserved aa regions observed in other GS. Northern blot analyses show that the GS mRNA is present in the sea urchin egg and is developmentally regulated in the embryo.

引用

页码：205 / 208

页数：4

共 16 条

[1] HOMOLOGOUS NUCLEAR GENES ENCODE CYTOPLASMIC AND MITOCHONDRIAL GLUTAMINE-SYNTHETASE IN DROSOPHILA-MELANOGASTER [J].