CHARACTERIZATION OF THE CHROMOSOMAL AAC(6')-II GENE-SPECIFIC FOR ENTEROCOCCUS-FAECIUM

被引:147
作者
COSTA, Y
GALIMAND, M
LECLERCQ, R
DUVAL, J
COURVALIN, P
机构
[1] INST PASTEUR,CNRS,UNITE AGENTS ANTIBACTERIENS,EP J0058,F-75724 PARIS 15,FRANCE
[2] UNIV PARIS SUD,HOP BICETRE,SERV BACTERIOL VIROL HYG,F-94275 LE KREMLIN BICETR,FRANCE
[3] HOP HENRI MONDOR,SERV BACTERIOL VIROL HYG,F-94010 CRETEIL,FRANCE
关键词
D O I
10.1128/AAC.37.9.1896
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Chromosomal gene aac(6')-Ii of Enterococcus faecium CEP 54-32, encoding a 6'-N-aminoglycoside acetyl-transferase was characterized. The gene was identified as a coding sequence of 549 bp corresponding to a protein with a calculated mass of 20,666 Da. Analysis of the sequence of the deduced protein suggested that it was the second member of a subfamily of AAC(6')-I enzymes. Insertional inactivation of aac(6')-Ii led to aminoglycoside susceptibility of CIP 54-32, suggesting that this gene plays a role in resistance to AAC(6')-I substrates. The gene was detected by DNA hybridization in all 26 strains of E. faecium tested but not in 44 other enterococci of 13 species. These data suggest that the aac(6')-Ii gene is species specific and may be used to identify E. faecium.
引用
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页码:1896 / 1903
页数:8
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