COMPARISON OF EXCRETORY SECRETORY AND CIRCULATING ANTIGENS OF TOXOPLASMA-GONDII BY ENZYME-IMMUNOASSAY AND IMMUNOBLOTTING

被引：8
作者：
HAFID, J
RABERIN, H
POZZETTO, B
AKONO, ZY
SUNG, RTM
JANA, M
GAUDIN, O
机构：
[1] Laboratoire de Parasitologie, Hôpital Nord, Saint-Etienne
来源：
INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1992年 / 22卷 / 08期
关键词：
TOXOPLASMA-GONDII; EXCRETORY SECRETORY ANTIGENS; CIRCULATING ANTIGENS; CAPTURE-ELISA; IMMUNOBLOTTING; CELL CULTURE;
D O I：
10.1016/0020-7519(92)90028-J
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Toxoplasma gondii trophozoites (RH strain) were cultured in embryonic fibroblasts in order to study the kinetics of production of excretory/secretory antigens, and the results were compared to the production of circulating antigens in an in vivo mouse model. By capture-ELISA, excretory/secretory antigens were first detected on the fourth day of culture whereas circulating antigens were first detected 1 day after infection. Similar concentrations of antigens were detected in both models as evidenced by comparable absorbance values. By immunoblotting, the excretory/secretory antigens were also detected later compared to circulating antigens (day 4 vs day 1). Seven major polypeptides were detected in both antigen preparations, six of them having the same molecular mass (110, 75, 48, 30, 24 and 22 kDa).
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页码：1083 / 1088
页数：6
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