DETERMINATION OF SOME ANTIOXIDANTS BY DERIVATIVE SPECTROPHOTOMETRIC METHOD AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The UV-derivative spectrophotometric method was used for assaying butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and propyl gallate (PG) in pure state and in laboratory mixtures. In this paper the results are presented using zero-crossing technique. In this way, the interference of two components mixed together has been eliminated. That is the greatest advantage of derivative spectroscopy compared with the classical UV-spectrophotometric method. After analysing the criteria for the application of this method, the amounts of these substances were determined. Simultaneously, a high-performance liquid chromatography was used.