EFFECT OF STORAGE TIME ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS IN BLOOD BAGS CONTAINING CPDA-1 ANTICOAGULANT DURING 30 DAYS OF STORAGE

Background/aim: Blood is used for transfusion purpose in transfusion medicine. Blood is stored at proper temperature using anticoagulants for maximum validity. But certain progressive changes occur in biochemical and hematological parameters of blood, which can cause harm to the patients. Therefore, the purpose of this study was to determine the changes occur in hematological and biochemical parameters of blood during 30-days of storage. Materials and methods: This prospective cohort study was conducted at Hayatabad Medical Complex Peshawar. We collected 450ml blood from 40 healthy voluntary donors in CPDA-1 anticoagulant. Collected blood was screened for transfusion transmissible infections. Fifty ml blood was transferred in another bag in a closed system. On 1st day, 15th day and 30th day of collection, hematological and biochemical parameters were determined and the data were analyzed through SPSS software using an ANOVA test. Results: The data indicated that during storage significant changes occurs in W C (P=.000), hemoglobin (P=.002), red blood cell count (P=.023), MCHC (P=.021) and platelets (P=.000) while in MCH (P=.216) and MCV (P=.693) no changes was observed. In biochemical parameters significant changes were observed in Na (P=.000), K (P=.000) and Cl (P=.023). Conclusion: We concluded that during storage significant changes were observed in hematological parameters i.e. hemoglobin, RBC, red cell indices and platelet count. In biochemical parameters significant changes were observed in sodium, potassium and chlorides. Therefore, for cardiovascular and renal failure patients fresh blood would be recommended to avoid adverse transfusion reaction.