A MICRO-METHOD FOR QUANTITATIVE ESTIMATION OF RENNETS AND OTHER PROTEOLYTIC ENZYMES

被引：45

作者：

LAWRENCE, RC

SANDERSO.WB

机构：

[1] New Zealand Dairy Research Institute, Palmerston North

来源：

JOURNAL OF DAIRY RESEARCH | 1969年 / 36卷 / 01期

关键词：

D O I：

10.1017/S0022029900012498

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

An agar diffusion slide assay using a thin layer of calcium caseinate has been developed as a micro-method for the quantitative study of calf rennet and other proteolytic enzymes. The number and width of the precipitation zones formed as a result of proteolytic activity depended upon the enzyme used, the concentration of calcium ion, pH and temperature. The assay was highly reproducible and particularly suitable for the measurement of large numbers of samples. It was considerably more sensitive than the milk clotting technique and, in general, more sensitive than other standard methods for measuring proteolytic activity. © 1969, Proprietors of Journal of Dairy Research. All rights reserved.

引用

页码：21 / &

共 11 条

[1]

BERRIDGE NJ, 1952, ANALYST, V77, P57, DOI 10.1039/an952770057b

[2]

BERRIDGE NJ, 1951, ENZYMES 2, V1, P107

[3] ACTION OF RENNET AND OTHER PROTEOLYTIC ENZYMES ON CASEIN IN CASEIN-AGAR GELS [J].