A RAPID STAINING TECHNIQUE FOR DETECTION OF RNASE AFTER POLYACRYLAMIDE GEL ELECTROPHORESIS

被引：76

作者：

WILSON, CW

机构：

[1] Department of Agronomy, University of Illinois, Urbana

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 31卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90294-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribonucleases may be detected in polyacrylamide gels after electrophoresis by incubation in a solution of RNA, followed by a 30 second dip into 0.2% toluidine blue. The method is very rapid and is sensitive to small amounts of plant RNases. The enzyme may be eluted from the gel after it is located by staining. © 1969.

引用

页码：506 / &

共 11 条

[1] DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].