DISTRIBUTION OF ALCOHOL AND SORBITOL DEHYDROGENASES - ASSESSMENT OF MESSENGER-RNA SPECIES IN MAMMALIAN-TISSUES

被引：74

作者：

ESTONIUS, M ^{[1
]}

DANIELSSON, O ^{[1
]}

KARLSSON, C ^{[1
]}

PERSSON, H ^{[1
]}

JORNVALL, H ^{[1
]}

HOOG, JO ^{[1
]}

机构：

[1] KAROLINSKA INST,MOLEC NEUROBIOL LAB,S-10401 STOCKHOLM 60,SWEDEN

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 215卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb18059.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tissue distribution of mRNA of alcohol dehydrogenases of classes I, II and III, and sorbitol dehydrogenase, was studied. mRNA from 19 different rat tissues was purified and analyzed by Northern blots, utilizing cDNA probes specific for the four dehydrogenases. Class-I alcohol-dehydrogenase mRNA was shown to be of widespread occurrence, detectable in all tissues including brain, but with pronounced differences in amounts. Hybridization revealed the pattern of occurrence of class-II alcohol-dehydrogenase mRNA to be unique, with transcripts only in the liver, duodenum, kidney, stomach, spleen and testis. Abundant levels of class-III alcohol-dehydrogenase (glutathione-dependent formaldehyde dehydrogenase) mRNA were present in all tissues analyzed, reflecting the general need for scavenging of formaldehyde in physiological cytoprotection. Sorbitol dehydrogenase mRNA was detected in all tissues except small intestine, in agreement with sorbitol resorbtion by passive diffusion in this tissue. In addition, evidence for a sex-specific expression, in the liver, of class-II alcohol dehydrogenase was obtained.

引用

页码：497 / 503

页数：7

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