SURVIVAL OF AEROMONAS-SALMONICIDA IN LAKE WATER

被引：66

作者：

MORGAN, JAW ^{[1
]}

CRANWELL, PA ^{[1
]}

PICKUP, RW ^{[1
]}

机构：

[1] INST FRESHWATER ECOL, AMBLESIDE LA22 OLP, CUMBRIA, ENGLAND

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 06期

关键词：

D O I：

10.1128/AEM.57.6.1777-1782.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The survival of Aeromonas salmonicida subsp. salmonicida in lake water was investigated by using a variety of techniques. They included acridine orange epifluorescence, respiration, cell culture, cell revival, flow cytometry, plasmid maintenance, and membrane fatty acid analysis. During a 21-day study, A. salmonicida became nonculturable in sterile lake water samples. Flow cytometry and direct microscopy indicated that cells were present. Although the nonculturable cells could not be revived, the recovery method did indicate that the presence of low numbers of culturable cells within samples could produce misleading results. Plasmid DNA, genomic DNA, and RNA were maintained in the nonculturable cells; in addition, changes in the fatty acid profiles were also detected. Although viability could not be proven, it was shown that the morphological integrity of nonculturable cells was maintained.

引用

页码：1777 / 1782

页数：6

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