AMINO-ACID-ANALYSIS OF PROTEIN HYDROLYSATES BY LITHIUM BUFFER SYSTEM

被引：0
作者：
SUWAN, S [1 ]
机构：
[1] CHULALONGKORN UNIV,CTR SCI & TECHNOL RES EQUIPMENT,BANGKOK 10330,THAILAND
来源：
JOURNAL OF THE SCIENCE SOCIETY OF THAILAND | 1990年 / 16卷 / 02期
关键词：
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A chromatographic procedure for the analysis of amino acid hydrolysates using lithium buffers and a dedicated amino acid analyzer is described. When used with an Hitachi amino acid analyzer model 835-50, the modified system permitted separation to near baseline of 18 common amino acids, plus another 6 amino compounds commonly encountered in such assays. Elution time from injection to arginine was 90 minutes. A major advantage of this system when only one amino acid analyzer is available is the greatly reduced work load compared to the classical switching over between sodium-based and lithium-based systems.
引用
收藏
页码：97 / 103
页数：7
相关论文
共 50 条
[21]   CYSTEINE - A POTENTIAL SOURCE OF ERROR IN AMINO-ACID-ANALYSIS OF MERCAPTOETHANE SULFONIC OR HYDROCHLORIC-ACID HYDROLYSATES OF PROTEINS AND PEPTIDES [J].
GARDNER, MLG .
ANALYTICAL BIOCHEMISTRY, 1984, 141 (02) :429-431
[22]   CITRULLINATED HISTIDINE-RICH PROTEIN - AMINO-ACID-ANALYSIS AND HISTOCHEMICAL DETECTION [J].
CHA, SH ;
TAKAHARA, H ;
EPSTEIN, WL ;
FUKUYAMA, K .
CLINICAL RESEARCH, 1993, 41 (01) :A61-A61
[23]   LOWRY PROTEIN DETERMINATION ON MEMBRANE PREPARATIONS - NEED FOR STANDARDIZATION BY AMINO-ACID-ANALYSIS [J].
PETERS, WHM ;
FLEURENJAKOBS, AMM ;
KAMPS, KMP ;
DEPONT, JJHHM ;
BONTING, SL .
ANALYTICAL BIOCHEMISTRY, 1982, 124 (02) :349-352
[24]   CHROMATOGRAPHIC AMINO-ACID-ANALYSIS OF PROTEIN HYDROLYZED IN POLYACRYLAMIDE AFTER REMOVAL OF AMMONIA [J].
FARMER, EE ;
UCHYTIL, TF ;
HELGESON, JP ;
DURBIN, RD .
CHROMATOGRAPHIA, 1987, 23 (02) :115-117
[25]   LITHIUM BUFFER SYSTEM FOR ACCELERATED SINGLE-COLUMN AMINO ACID ANALYSIS IN PHYSIOLOGICAL FLUIDS [J].
KEDENBURG, CP .
ANALYTICAL BIOCHEMISTRY, 1971, 40 (01) :35-+
[26]   HIGH-RESOLUTION AMINO-ACID-ANALYSIS [J].
ROSSI, D ;
TRISCIANI, A .
INTERNATIONAL LABORATORY, 1980, 10 (05) :31-&
[27]   INTERFERENCE OF CARBOHYDRATES IN THE QUANTITATION OF PROTEIN-BOUND CITRULLINE BY AMINO-ACID-ANALYSIS [J].
KOSHY, KM ;
BOGGS, JM .
ANALYTICAL BIOCHEMISTRY, 1993, 208 (02) :375-381
[28]   PRACTICAL APPLICATION OF HPLC TO AMINO-ACID-ANALYSIS [J].
PFEIFER, R ;
KAROL, R ;
KORPI, J ;
BURGOYNE, R ;
MCCOURT, D .
AMERICAN LABORATORY, 1983, 15 (03) :78-&
[29]   VIGABATRIN INTERFERENCE WITH URINARY AMINO-ACID-ANALYSIS [J].
PREECE, MA ;
SEWELL, IJ ;
TAYLOR, JA ;
GREEN, A .
CLINICA CHIMICA ACTA, 1993, 218 (01) :113-116
[30]   Chemometric Analysis of the Amino Acid Requirements of Antioxidant Food Protein Hydrolysates [J].
Udenigwe, Chibuike C. ;
Aluko, Rotimi E. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 12 (05) :3148-3161
12345