AN ELISA FOR THE DETECTION OF LOCALIZED ADHERENT CLASSIC ENTEROPATHOGENIC ESCHERICHIA-COLI SEROGROUPS

被引：0
作者：
ALBERT, MJ
ANSARUZZAMAN, M
FARUQUE, SM
NEOGI, PKB
HAIDER, K
TZIPORI, S
机构：
来源：
JOURNAL OF INFECTIOUS DISEASES | 1991年 / 164卷 / 05期
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D O I：
暂无
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
An ELISA for the detection of classic enteropathogenic Escherichia coli (EPEC) serogroups was developed. It detected EPEC positive for localized adherence (LA) by HeLa cell assay and EPEC positive for EPEC adherence factor (EAF) by DNA probe assay. A specific antiserum was raised with LA+ EPEC strain E2348/69 (serotype O127:H6) by immunizing rabbits and then absorbing the antiserum with its LA- derivative, MAR20. The absorbed antiserum reacted specifically with all 90 strains of E. coli belonging to eight different EPEC serogroups that were LA+ by HeLa cell assay and EAF+ by DNA probe assay. All E. coli strains including EPEC serogroups that were LA- by HeLa cell assay and EAF- by DNA probe assay were also negative by ELISA. Thus the ELISA is 100% sensitive and specific in detecting LA+ classic EPEC serogroups.
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页码：986 / 989
页数：4
相关论文
共 15 条
[1]   LOCALIZATION OF A DETERMINANT FOR HEP-2 ADHERENCE BY ENTEROPATHOGENIC ESCHERICHIA-COLI [J].
BALDINI, MM ;
NATARO, JP ;
KAPER, JB .
INFECTION AND IMMUNITY, 1986, 52 (01) :334-336
[2]   A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].
FEINBERG, AP ;
VOGELSTEIN, B .
ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13
[3]  
GOMES TAT, 1989, J INFECT DIS, V160, P131
[4]   ROLE OF PLASMID-ENCODED ADHERENCE FACTORS IN ADHESION OF ENTEROPATHOGENIC ESCHERICHIA-COLI TO HEP-2 CELLS [J].
KNUTTON, S ;
BALDINI, MM ;
KAPER, JB ;
MCNEISH, AS .
INFECTION AND IMMUNITY, 1987, 55 (01) :78-85
[5]   ACTIN ACCUMULATION AT SITES OF BACTERIAL ADHESION TO TISSUE-CULTURE CELLS - BASIS OF A NEW DIAGNOSTIC-TEST FOR ENTEROPATHOGENIC AND ENTEROHEMORRHAGIC ESCHERICHIA-COLI [J].
KNUTTON, S ;
BALDWIN, T ;
WILLIAMS, PH ;
MCNEISH, AS .
INFECTION AND IMMUNITY, 1989, 57 (04) :1290-1298
[6]   USE OF DNA PROBES AND HEP-2 CELL ADHERENCE ASSAY TO DETECT DIARRHEAGENIC ESCHERICHIA-COLI [J].
LEVINE, MM ;
PRADO, V ;
ROBINSBROWNE, R ;
LIOR, H ;
KAPER, JB ;
MOSELEY, SL ;
GICQUELAIS, K ;
NATARO, JP ;
VIAL, P ;
TALL, B .
JOURNAL OF INFECTIOUS DISEASES, 1988, 158 (01) :224-227
[7]   THE DIARRHEAL RESPONSE OF HUMANS TO SOME CLASSIC SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA-COLI IS DEPENDENT ON A PLASMID ENCODING AN ENTEROADHESIVENESS FACTOR [J].
LEVINE, MM ;
NATARO, JP ;
KARCH, H ;
BALDINI, MM ;
KAPER, JB ;
BLACK, RE ;
CLEMENTS, ML ;
OBRIEN, AD .
JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) :550-559
[8]   ESCHERICHIA-COLI THAT CAUSE DIARRHEA - ENTEROTOXIGENIC, ENTEROPATHOGENIC, ENTEROINVASIVE, ENTEROHEMORRHAGIC, AND ENTEROADHERENT [J].
LEVINE, MM .
JOURNAL OF INFECTIOUS DISEASES, 1987, 155 (03) :377-389
[9]   ATTACHING AND EFFACING ACTIVITIES OF RABBIT AND HUMAN ENTEROPATHOGENIC ESCHERICHIA-COLI IN PIG AND RABBIT INTESTINES [J].
MOON, HW ;
WHIPP, SC ;
ARGENZIO, RA ;
LEVINE, MM ;
GIANNELLA, RA .
INFECTION AND IMMUNITY, 1983, 41 (03) :1340-1351
[10]   DETECTION OF AN ADHERENCE FACTOR OF ENTEROPATHOGENIC ESCHERICHIA-COLI WITH A DNA PROBE [J].
NATARO, JP ;
BALDINI, MM ;
KAPER, JB ;
BLACK, RE ;
BRAVO, N ;
LEVINE, MM .
JOURNAL OF INFECTIOUS DISEASES, 1985, 152 (03) :560-565
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