PARTIAL-PURIFICATION AND SOME PROPERTIES OF ALCOHOL ACYLTRANSFERASE FROM STRAWBERRY FRUITS

被引：78

作者：

PEREZ, AG ^{[1
]}

SANZ, C ^{[1
]}

OLIAS, JM ^{[1
]}

机构：

[1] CSIC,INST GRASA & DERIVADOS,UEI FISIOL & TECNOL POST RECOLECC,APDO 1078,E-41012 SEVILLE,SPAIN

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1993年 / 41卷 / 09期

关键词：

D O I：

10.1021/jf00033a021

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The enzyme system concerning volatile ester formation in strawberry Fragaria ananassa x Duchessne var. Chandler was studied. Protein with alcohol acyltransferase activity was purified about 29-fold from Chandler strawberry fruits by ammonium sulfate fractionation, gel filtration, and anion-exchange chromatography. The enzyme activity had a pH optimum of 8.0 and an optimum temperature of 35-degrees-C. The apparent M(r) estimated by gel filtration was 70 000. The enzyme was tested for its preference in using different acyl-CoAs and alcohols. Maximum activity was obtained using acetyl-CoA and hexyl alcohol as substrates. A clear correlation was observed between substrate preference and volatile esters present in strawberry var. Chandler.

引用

页码：1462 / 1466

页数：5

共 26 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] ACYL COENZYME-A PREFERENCE OF DIACYLGLYCEROL ACYLTRANSFERASE FROM THE MATURING SEEDS OF CUPHEA, MAIZE, RAPESEED, AND CANOLA [J].

CAO, YZ ;

HUANG, AHC .

PLANT PHYSIOLOGY, 1987, 84 (03) :762-765

[3] SOLUBILITY OF PALMITOYL-COENZYME-A IN ACYLTRANSFERASE ASSAY BUFFERS CONTAINING MAGNESIUM-IONS [J].

CONSTANTINIDES, PP ;

STEIM, JM .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1986, 250 (01) :267-270

[4] AROMA QUALITY EVALUATION OF TOMATOES, APPLES, AND STRAWBERRIES [J].

DIRINCK, P ;

SCHREYEN, L ;

SCHAMP, N .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1977, 25 (04) :759-763

[5]

DRAWERT F, 1982, Z NATURFORSCH C, V37, P849

[6] PURIFICATION AND SOME PROPERTIES OF ALCOHOL ACETYLTRANSFERASE FROM BANANA FRUIT [J].

HARADA, M ;

UEDA, Y ;

IWATA, T .

PLANT AND CELL PHYSIOLOGY, 1985, 26 (06) :1067-1074

[7] CELL-FREE SYNTHESIS OF ETHYL-ACETATE BY EXTRACTS FROM SACCHAROMYCES-CEREVISIAE [J].

HOWARD, D ;

ANDERSON, RG .

JOURNAL OF THE INSTITUTE OF BREWING, 1976, 82 (02) :70-71

[8]

LANZA E, 1976, J AGR FOOD CHEM, V82, P1247

[9] PRODUCTION OF VOLATILES BY RIPENING BANANAS [J].

MACKU, C ;

JENNINGS, WG .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1987, 35 (05) :845-848

[10] L-LEUCINE AS A PRECURSOR OF ISOAMYL ALCOHOL AND ISOAMYL ACETATE, VOLATILE AROMA CONSTITUENTS OF BANANA FRUIT DISCS [J].

MYERS, MJ ;

ISSENBERG, P ;

WICK, EL .

PHYTOCHEMISTRY, 1970, 9 (08) :1693-+

← 1 2 3 →