GLUTATHIONE RELEASE BY PULMONARY ALVEOLAR MACROPHAGES IN RESPONSE TO PARTICLES INVITRO

被引：35
作者：
BOEHME, DS
MAPLES, KR
HENDERSON, RF
机构：
来源：
TOXICOLOGY LETTERS | 1992年 / 60卷 / 01期
关键词：
MACROPHAGES; QUARTZ; INVITRO; GLUTATHIONE; CROCIDOLITE ASBESTOS;
D O I：
10.1016/0378-4274(92)90046-M
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
We studied whether cultured rat pulmonary alveolar macrophages (PAM) could release glutathione (GSH) in response to latex beads, quartz, and crocidolite asbestos. PAM were exposed for 2 h to 0-100-mu-g of particles per 1 x 10(6) cells. Both quartz and asbestos produced concentration-dependent increases (up to 8-10-fold) in the amount of GSH recovered in the medium and decreases in the cellular GSH levels. In contrast, latex beads did not produce any changes in GSH levels. We also measured lactate dehydrogenase (LDH) levels as an index of toxicity. Only quartz and asbestos were able to produce increases in LDH levels in the medium. The release of GSH occurred at particle concentrations that did not cause release of LDH. Our results indicated that, in vitro, PAM release GSH in response to toxic particles.
引用
收藏
页码：53 / 60
页数：8
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