MEASUREMENT OF KINETIC BINDING CONSTANTS OF A PANEL OF ANTI-SAPORIN ANTIBODIES USING A RESONANT MIRROR BIOSENSOR

被引：91

作者：

GEORGE, AJT ^{[1
]}

FRENCH, RR ^{[1
]}

GLENNIE, MJ ^{[1
]}

机构：

[1] SOUTHAMPTON GEN HOSP, TENOVUS RES LABS, SOUTHAMPTON SO9 4XY, HANTS, ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 183卷 / 01期

关键词：

KINETIC DETERMINATION; RESONANT MIRROR BIOSENSOR; BIOSENSOR; SAPORIN; TOXIN; AFFINITY; BISPECIFIC ANTIBODY;

D O I：

10.1016/0022-1759(95)00031-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used a resonant mirror biosensor to determine the kinetics of binding of four antibodies, and their Fab' fragments, to their antigen, the plant-derived ribosome-inactivating protein (RIP) saporin. The analysis of the affinity of the antibodies was in reasonable agreement with values obtained by conventional techniques. However, the kinetic data showed that all four antibodies have a high dissociation rate constant (k(diss)). These antibodies have been used in the construction of bispecific antibodies used to deliver saporin to tumour cells, and it is highly probable that the in vivo efficacy of the bispecific antibodies is limited by the high rate of dissociation of antibody-toxin complexes.

引用

页码：51 / 63

页数：13

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