REGULATION OF THE INDUCTION OF ALKALINE-PHOSPHATASE IN CHORIOCARCINOMA CELLS BY SODIUM-BUTYRATE

被引：16
作者：
CHOU, JY
机构：
[1] Laboratory of Biomedical Sciences, National Institutes of Child Health and Human Development, National Institutes of Health, Bethesda, 20014, Maryland, Building 6
来源：
IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION | 1979年 / 15卷 / 10期
关键词：
alkaline phosphatase; choriocarcinoma cells; sodium butyrate;
D O I：
10.1007/BF02618305
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
Alkaline phosphatase is induced in human choriocarcinoma cells by short-chain fatty acids, especially sodium butyrate. This fatty acid increases the phosphatase activity immediately and in a nearly linear fashion. Only phosphatase with an alkaline pH optimum is induced. Both the induced alkaline phosphatase and the basal enzyme are precipitated by antiserum against term-placental alkaline phosphatase, but the choriocarcinoma phosphatase is less stable to heating than is the term-placental enzyme. The induction of alkaline phosphatase activity requires cellular synthesis of protein, RNA and DNA. The regulation of induction probably occurs at the transcriptional level. © 1979 Tissue Culture Association.
引用
收藏
页码：789 / 795
页数：7
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