SOME FACTORS AFFECTING SOMATIC EMBRYOGENESIS EFFICIENCY IN SOYBEAN (GLYCINE-MAX (L) MERR)

被引：3
作者：
GRIGA, M
机构：
[1] Research Institute of Technical Crops and Legumes, Oseva, Šumperk
来源：
BIOLOGIA PLANTARUM | 1993年 / 35卷 / 02期
关键词：
D O I：
10.1007/BF02925936
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Selected factors affecting somatic embryogenesis efficiency have been studied, namely genotype, explant type and its orientation in the medium, different basal media, different auxins for somatic embryo induction, and two ways of donor plant cultivation. The key role is played by genotype and auxin used, the minimum effect was observed due to basal media. In the series of subsequent experiments we have found the best combination of individual factors as follows: cv, Altona, 10 muM 2,4-D, L2 basal medium, central part of immature cotyledon as initial explant oriented by adaxial side down on the agar medium, and field grown donor plants. This combination exhibited 100 % embryogenic explants with 5.43 +/- 0.65 somatic embryos per explant, i. e. somatic embryogenesis efficiency 5.43.
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收藏
页码：179 / 189
页数：11
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