PROCEDURES FOR SAMPLING DEEP SUBSURFACE MICROBIAL COMMUNITIES IN UNCONSOLIDATED SEDIMENTS

被引：39

作者：

RUSSELL, BF

PHELPS, TJ

GRIFFIN, WT

SARGENT, KA

机构：

来源：

GROUND WATER MONITORING AND REMEDIATION | 1992年 / 12卷 / 01期

关键词：

D O I：

10.1111/j.1745-6592.1992.tb00414.x

中图分类号：

TV21 [水资源调查与水利规划];

学科分类号：

081501 ;

摘要：

A corehole sampling project utilizing a wireline coring system provided sediment samples for microbiological characterization from deep unconsolidated sediments. Sampling tools were developed or modified to minimize contamination during sample acquisition and to facilitate stringent decontamination requirements. Quality assurance procedures, including the use of tracers, were implemented to minimize and quantify contamination from drilling hardware, drilling fluids and sample processing. Tracers included microspheres, potassium bromide, rhodamine dye, and perfluorocarbons, which enabled the detection and measurement of 1 mg of drilling fluid per kg of sediment. In addition, sample processing was performed on-site in an anaerobic chamber to prevent exposure of the subsurface materials to atmospheric oxygen concentrations. Sediment samples were then disbursed to investigators at National Laboratories and universities funded through the Department of Energy Subsurface Science Microbiology Program for microbiological characterization. Results of these efforts demonstrated that representative subsurface samples were collected and disbursed.

引用

页码：96 / 104

页数：9

共 10 条

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