DDAVP FACTOR-VIII CONCENTRATE AND ITS PROPERTIES INVIVO AND INVITRO

DDAVP fraction I-0 was prepared from a plasma pool derived from 80 blood donors who had received DDAVP and tranexamic acid. A control fraction I-0 was prepared from a plasma pool from the same group of blood donors under identical conditions except for the drug treatment. The DDAVP plasma pool as well as the DDAVP fraction I-0 contained 2-3 times as much VIII:C as the controls. VIIIR:Ag increased to a lesser extent. No difference in stability of VIII:C was seen. In vivo studies showed that infusion of the DDAVP fraction I-0 to 3 patients with severe haemophilia A (<0.01 i.u. VIII:C/ml) caused a 2-3 times higher increase in VIII:C than after infusion of the same volume of the control fraction I-0. No difference in disappearance rate of VIII:C was seen. Both factor VIII concentrates normalised the prolonged bleeding time and caused a secondary VIII:C increase in VWD. It is concluded that administration of DDAVP to blood donors prior to collection of blood increases the content of VIII:C in the ensuing factor VIII concentrate and secondly, that such stimulation has no demonstrable qualitative effect on the factor VIII obtained. © 1979.