INHIBITION OF GROWTH AND CYSTEINE PROTEINASE ACTIVITY OF STAPHYLOCOCCUS-AUREUS V8 BY PHOSPHORYLATED CYSTATIN-ALPHA IN SKIN CORNIFIED ENVELOPE

被引：44
作者：
TAKAHASHI, M
TEZUKA, T
KATUNUMA, N
机构：
[1] KINKI UNIV,SCH MED,DEPT DERMATOL,OSAKA 589,JAPAN
[2] TOKUSHIMA BUNRI UNIV,INST HLTH SCI,YAMASHIRO,TOKUSHIMA 770,JAPAN
来源：
FEBS LETTERS | 1994年 / 355卷 / 03期
关键词：
PHOSPHORYLATED CYSTATIN ALPHA; CORNIFIED ENVELOPE; EPIDERMAL BARRIER FUNCTION; STAPHYLOCOCCUS AUREUS V8;
D O I：
10.1016/0014-5793(94)01196-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The activity of a cysteine proteinase purified from Staphylacoccus aureus V8 (SAV8) was inhibited by phosphorylated cystatin alpha (P-cystatin alpha) and by purified cornified envelope protein of newborn rat, a conjugated form of P-cystatin alpha. Immunohistochemical analysis demonstrated a marked decrease in P-cystatin alpha content in cornified envelope treated with sphingosine. The inhibition of papain activity by proteins from sphingosine-treated skin was much weaker than that exerted by proteins from the untreated skin. The suppression of SAV8 colony formation inoculated on the sphingosine-treated skin was examined. Colony formation on the sphingosine-treated skin was enhanced compared to that on normal skin. These findings suggest that P-cystatin alpha in the cornified envelope may have a bacteriostatic barrier function against bacterial infection, such as that with SAV8.
引用
收藏
页码：275 / 278
页数：4
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