ISOLATION OF RNA APTAMERS FOR BIOLOGICAL COFACTORS BY IN-VITRO SELECTION

被引：195

作者：

BURGSTALLER, P ^{[1
]}

FAMULOK, M ^{[1
]}

机构：

[1] UNIV MUNICH,INST BIOCHEM,FACHBEREICH CHEM,D-82152 MARTINSRIED,GERMANY

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH | 1994年 / 33卷 / 10期

关键词：

D O I：

10.1002/anie.199410841

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Short RNA sequences with specific secondary structure (see below) are the binding motifs for the cofactors flavin adenine dinucleotide and flavin mononucleotide (FAD and FMN, respectively), according to selection experiments with randomized RNA pools and FAD‐ and FMN‐modified agarose. It was also shown that only the flavin portion plays a role in binding. Analogous experiments were conducted with NAD+ and its mononucleotide. (Figure Presented.) Copyright © 1994 by VCH Verlagsgesellschaft mbH, Germany

引用

页码：1084 / 1087

页数：4

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