A MURINE IGG1 MONOCLONAL-ANTIBODY THAT BINDS SPECIFICALLY TO OUTER SURFACE PROTEIN-A OF LYME-DISEASE SPIROCHETE BORRELIA-BURGDORFERI

被引：1
作者：
ABOLHASSANI, M
PAVIA, CS
DUNN, JJ
CHIAO, JW
机构：
[1] NEW YORK MED COLL,DEPT MED,207 VOSBURGH PAVIL,VALHALLA,NY 10595
[2] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973
来源：
FEMS MICROBIOLOGY LETTERS | 1993年 / 109卷 / 2-3期
关键词：
LYME DISEASE; ANTI-OSPA; BORRELIA-BURGDORFERI;
D O I：
10.1111/j.1574-6968.1993.tb06185.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A murine monoclonal antibody, designated MA-2G9, directed against outer surface protein A (OspA) of the Lyme disease spirochete, Borrelia burgdorferi, has been produced. Antibody MA-2G9, IgG1 subclass, was purified by affinity chromatography on protein G Sepharose column and used for purification of OspA antigen from Borrelia burgdorferi cell lysate. Epitope specificity was studied by Western immunoblotting, using several strains of B. burgdorferi and non-Lyme disease bacteria such as Treponema pallidum and B. hermsii. The MA-2G9 monoclonal antibody reacted specifically with recombinant OspA as well as with native OspA in sonicated B. burgdorferi strains. No reaction was observed with T pallidum, Escherichia coli, Staphylococcus aureus and B. hermsii lysates. The MA-2G9 antibody also recognized the denatured form of OspA indicating that it is directed against sequential epitope and not conformational epitope.
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页码：303 / 309
页数：7
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