THE EFFECT OF THE UL42 PROTEIN ON THE DNA-POLYMERASE-ACTIVITY OF THE CATALYTIC SUBUNIT OF THE DNA-POLYMERASE ENCODED BY HERPES-SIMPLEX VIRUS TYPE-1

被引：17
作者：
HART, GJ
BOEHME, RE
机构：
[1] Department of Virology, Glaxo Group Research Ltd., Greenford, Middlesex UB6 OHE, Greenford Road
来源：
FEBS LETTERS | 1992年 / 305卷 / 02期
关键词：
DNA POLYMERASE; UL42; PROTEIN; HERPES SIMPLEX VIRUS;
D O I：
10.1016/0014-5793(92)80872-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect that the UL42 protein of herpes simplex virus type 1 has on the DNA polymerase activity of the DNA polymerase catalytic subunit (Pol) of the same virus has been investigated. The observed effects are critically dependent on the salt used and its concentration, such that the UL42 protein may inhibit, have little or no effect on, or activate the Pol activity, depending on the conditions used. The observed effects are due to the values for K(mapp) for activated DNA and V(maxapp) for Pol and the Pol UL42 protein complex differently varying.with salt concentration.
引用
收藏
页码：97 / 100
页数：4
相关论文
共 12 条
[1]   STRUCTURE AND FUNCTION OF THE SACCHAROMYCES-CEREVISIAE CDC2 GENE ENCODING THE LARGE SUBUNIT OF DNA POLYMERASE-III [J].
BOULET, A ;
SIMON, M ;
FAYE, G ;
BAUER, GA ;
BURGERS, PMJ .
EMBO JOURNAL, 1989, 8 (06) :1849-1854
[2]  
CHALLBERG MD, 1989, ANNU REV BIOCHEM, V58, P671
[3]  
CRUTE JJ, 1989, J BIOL CHEM, V264, P19266
[4]   THE ESSENTIAL 65-KILODALTON DNA-BINDING PROTEIN OF HERPES-SIMPLEX VIRUS STIMULATES THE VIRUS-ENCODED DNA-POLYMERASE [J].
GALLO, ML ;
DORSKY, DI ;
CRUMPACKER, CS ;
PARRIS, DS .
JOURNAL OF VIROLOGY, 1989, 63 (12) :5023-5029
[5]   THE HERPES-SIMPLEX VIRUS TYPE-1 UL42 GENE-PRODUCT - A SUBUNIT OF DNA-POLYMERASE THAT FUNCTIONS TO INCREASE PROCESSIVITY [J].
GOTTLIEB, J ;
MARCY, AI ;
COEN, DM ;
CHALLBERG, MD .
JOURNAL OF VIROLOGY, 1990, 64 (12) :5976-5987
[6]  
HERNANDEZ TR, 1990, J BIOL CHEM, V265, P11227
[7]   ENZYMATIC-ACTIVITIES OF OVEREXPRESSED HERPES-SIMPLEX VIRUS-DNA POLYMERASE PURIFIED FROM RECOMBINANT BACULOVIRUS-INFECTED INSECT CELLS [J].
MARCY, AI ;
OLIVO, PD ;
CHALLBERG, MD ;
COEN, DM .
NUCLEIC ACIDS RESEARCH, 1990, 18 (05) :1207-1215
[8]   THE EVOLUTIONARY CONSERVATION OF DNA POLYMERASE-ALPHA [J].
MILLER, MA ;
KORN, D ;
WANG, TSF .
NUCLEIC ACIDS RESEARCH, 1988, 16 (16) :7961-7973
[9]   HERPES-SIMPLEX VIRUS TYPE-1 ORIGIN-DEPENDENT DNA-REPLICATION IN INSECT CELLS USING RECOMBINANT BACULOVIRUSES [J].
STOW, ND .
JOURNAL OF GENERAL VIROLOGY, 1992, 73 :313-321
[10]  
Summers MD, 1987, MANUAL METHODS BACUL
12