SYNTHESIS AND CHROMATOGRAPHY OF 1,2-DIAMINO-4,5-DIMETHOXYBENZENE, 6,7-DIMETHOXY-2-METHYLQUINOXALINE AND 6,7-DIMETHOXY-2,3-DIMETHYLQUINOXALINE FOR USE IN A LIQUID-CHROMATOGRAPHIC FLUOROMETRIC ASSAY OF METHYLGLYOXAL

被引：85

作者：

MCLELLAN, AC

THORNALLEY, PJ

机构：

[1] Department of Chemistry and Biological Chemistry, University of Essex, Colchester, CO4 3SQ, Wivenhoe Park

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 263卷 / 1-2期

关键词：

FLUOROMETRY; CHROMATOGRAPHY; BUTAN-2,3-DIONE; 1,2-DIAMINO-4,5-DIMETHOXYBENZENE; 6,7-DIAMINO-2-METHYLQUINOXALINE; 6,7-DIMETHOXY-2,3-DIMETHYLQUINOXALINE; METHYLGLYOXAL;

D O I：

10.1016/0003-2670(92)85435-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Methylglyoxal (2-oxopropanal) may be assayed in chemical and biological systems by derivatisation with 1,2-diamino-4,5-dimethoxybenzene to form 6,7-dimethoxy-2-methylquinoxaline. Methods are described to produce 1,2-diamino-4,5-dimethoxybenzene dihydrochloride (the derivatising agent), 6,7-dimethoxy-2-methylquinoxaline (the methylglyoxal adduct) and 6,7-dimethoxy-2,3-dimethylquinoxaline (an internal standard). Liquid chromatography data of the methylglyoxal adduct and internal standard with spectrophotometric and fluorimetric detection are presented. The purity of these compounds was suitable for their use in assay, recovery experiments and internal standardisation in the analysis of trace amounts of methylglyoxal.

引用

页码：137 / 142

页数：6

共 15 条

[1] IMPROVED PREPARATION AND STRUCTURAL CONFORMATION OF THE FLUORESCENCE LABELING REAGENTS 1,2-DIAMINO-4,5-DIMETHOXYBENZENE AND 3,4-DIHYDRO-6,7-DIMETHOXY-4-METHYL-3-OXO-QUINOXALINE-2-CARBONYL CHLORIDE [J].