SYNTHESIS AND CHROMATOGRAPHY OF 1,2-DIAMINO-4,5-DIMETHOXYBENZENE, 6,7-DIMETHOXY-2-METHYLQUINOXALINE AND 6,7-DIMETHOXY-2,3-DIMETHYLQUINOXALINE FOR USE IN A LIQUID-CHROMATOGRAPHIC FLUOROMETRIC ASSAY OF METHYLGLYOXAL

被引：85
作者：
MCLELLAN, AC
THORNALLEY, PJ
机构：
[1] Department of Chemistry and Biological Chemistry, University of Essex, Colchester, CO4 3SQ, Wivenhoe Park
来源：
ANALYTICA CHIMICA ACTA | 1992年 / 263卷 / 1-2期
关键词：
FLUOROMETRY; CHROMATOGRAPHY; BUTAN-2,3-DIONE; 1,2-DIAMINO-4,5-DIMETHOXYBENZENE; 6,7-DIAMINO-2-METHYLQUINOXALINE; 6,7-DIMETHOXY-2,3-DIMETHYLQUINOXALINE; METHYLGLYOXAL;
D O I：
10.1016/0003-2670(92)85435-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Methylglyoxal (2-oxopropanal) may be assayed in chemical and biological systems by derivatisation with 1,2-diamino-4,5-dimethoxybenzene to form 6,7-dimethoxy-2-methylquinoxaline. Methods are described to produce 1,2-diamino-4,5-dimethoxybenzene dihydrochloride (the derivatising agent), 6,7-dimethoxy-2-methylquinoxaline (the methylglyoxal adduct) and 6,7-dimethoxy-2,3-dimethylquinoxaline (an internal standard). Liquid chromatography data of the methylglyoxal adduct and internal standard with spectrophotometric and fluorimetric detection are presented. The purity of these compounds was suitable for their use in assay, recovery experiments and internal standardisation in the analysis of trace amounts of methylglyoxal.
引用
收藏
页码：137 / 142
页数：6
相关论文
共 15 条
  • [1] IMPROVED PREPARATION AND STRUCTURAL CONFORMATION OF THE FLUORESCENCE LABELING REAGENTS 1,2-DIAMINO-4,5-DIMETHOXYBENZENE AND 3,4-DIHYDRO-6,7-DIMETHOXY-4-METHYL-3-OXO-QUINOXALINE-2-CARBONYL CHLORIDE
    DAVE, KJ
    RILEY, CM
    VANDERVELDE, D
    STOBAUGH, JF
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1990, 8 (03) : 307 - 312
  • [2] 1,2-DIAMINO-4,5-METHYLENEDIOXYBENZENE AS A HIGHLY SENSITIVE FLUOROGENIC REAGENT FOR ALPHA-DICARBONYL COMPOUNDS
    HARA, S
    YAMAGUCHI, M
    TAKEMORI, Y
    YOSHITAKE, T
    NAKAMURA, M
    [J]. ANALYTICA CHIMICA ACTA, 1988, 215 (1-2) : 267 - 276
  • [3] HIGHLY SENSITIVE DETERMINATION OF N-ACETYL-NEURAMINIC AND N-GLYCOLYLNEURAMINIC ACIDS IN HUMAN-SERUM AND URINE AND RAT SERUM BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    HARA, S
    YAMAGUCHI, M
    TAKEMORI, Y
    NAKAMURA, M
    OHKURA, Y
    [J]. JOURNAL OF CHROMATOGRAPHY, 1986, 377 : 111 - 119
  • [4] DETERMINATION OF ALPHA-KETO ACIDS IN SERUM AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
    HARA, S
    TAKEMORI, Y
    YAMAGUCHI, M
    NAKAMURA, M
    OHKURA, Y
    [J]. JOURNAL OF CHROMATOGRAPHY, 1985, 344 (NOV): : 33 - 39
  • [5] FLUORIMETRIC DETERMINATION OF ALPHA-KETO ACIDS WITH 4,5-DIMETHOXY-1,2-DIAMINOBENZENE AND ITS APPLICATION TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    HARA, S
    TAKEMORI, Y
    IWATA, T
    YAMAGUCHI, M
    NAKAMURA, M
    OHKURA, Y
    [J]. ANALYTICA CHIMICA ACTA, 1985, 172 (JUN) : 167 - 173
  • [6] ANALYSIS OF METHYL GLYOXAL IN FOODS AND BEVERAGES
    HAYASHI, T
    SHIBAMOTO, T
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1985, 33 (06) : 1090 - 1093
  • [7] IWATA T, 1985, CHEM PHARM BULL, V33, P3499
  • [8] CONVENIENT QUANTITATIVE SYNTHESIS OF METHYLGLYOXAL FOR GLYOXALASE I ASSAYS
    KELLUM, MW
    ORAY, B
    NORTON, SJ
    [J]. ANALYTICAL BIOCHEMISTRY, 1978, 85 (02) : 586 - 590
  • [9] MARGUET J, 1987, TETRAHEDRON, V43, P351
  • [10] MCLELLAN AC, UNPUB ANAL BIOCH
12