ELECTROTRANSFORMATION OF STREPTOCOCCUS-PNEUMONIAE

被引：2

作者：

CANVIN, JR

ANDREW, PW

BOULNOIS, GJ

MITCHELL, TJ

机构：

[1] UNIV LEICESTER,DEPT MICROBIOL,POB 138,UNIV RD,LEICESTER LE1 9HN,ENGLAND

[2] LEICESTER ROYAL INFIRM,DEPT IMMUNOL,LEICESTER LE2 7LX,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 18卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00794.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conditions of electroporation to transform encapsulated strains of Streptococcus pneumoniae with plasmid DNA have been defined. For a heavily encapsulated strain, an electroporation solution of 10-20% (v/v) glycerol and 3.2 kV cm-1 field strength, 1000 OMEGA resistance and 25 muF capacitance were optimal. For lightly encapsulated or non-encapsulated strains, optimal conditions were a sucrose-based electroporation solution and 12.5 kV cm-1 field strength, 200 OMEGA resistance and 25 muF capacitance.

引用

页码：35 / 38

页数：4

共 20 条

[1]

ALLOW AY J. LIONEL, 1932, JOUR EXP MED, V55, P91, DOI 10.1084/jem.55.1.91

[2]

CAPARON MG, 1991, METHOD ENZYMOL, P580

[3] IMPROVED ELECTROPORATION AND CLONING VECTOR SYSTEM FOR GRAM-POSITIVE BACTERIA [J].