STRUCTURE OF THE PROMOTER REGION OF THE GENE ENCODING CYTOCHROME-C-OXIDASE SUBUNIT-V IN DICTYOSTELIUM

被引：3
|
作者：
RIZZUTO, R
SANDONA, D
BRINI, M
MARSCHALEK, R
DINGERMANN, T
BISSON, R
机构：
[1] UNIV PADUA,DIPARTIMENTO SCI BIOMED SPERTMENTALI,PATOL & BIOL MOLEC LAB,VIA TRIESTE 75,I-35121 PADUA,ITALY
[2] UNIV ERLANGEN NURNBERG,FAK MED,INST BIOCHEM,W-8520 ERLANGEN,GERMANY
[3] UNIV PADUA,CNR,CTR STUDI FISIOL MITOCONDRI,I-35100 PADUA,ITALY
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 211卷 / 03期
关键词：
D O I：
10.1111/j.1432-1033.1993.tb17564.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA for the nuclear-encoded subunit V of Dictyostelium discoideum cytochrome-c oxidase was used as a probe to screen a genomic library and isolate the complete gene. Primer-extension analysis revealed two transcription start sites located 32 and 39 nucleotides upstream of the translation initiation codon. The chloramphenicol acetyltransferase assay in transient and stable Dictyostelium transformants indicated that the 400-bp dT-rich segment 5' to the transcription start sites retained promoter activity. This region contains in octanucleotide sequence similar to the yeast HAP2/3/4 responsive element.
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页码：411 / 414
页数：4
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