THE SECONDARY STRUCTURE OF THE FERREDOXIN TRANSIT SEQUENCE IS MODULATED BY ITS INTERACTION WITH NEGATIVELY CHARGED LIPIDS

被引：33
作者：
HORNIAK, L
PILON, M
VANTHOF, R
DEKRUIJFF, B
机构：
[1] UNIV UTRECHT, DEPT MOLEC CELL BIOL, PADUALAAN 8, 3584 CH UTRECHT, NETHERLANDS
[2] COMENIUS UNIV BRATISLAVA, INST MOLEC & SUBCELLULAR BIOL, BRATISLAVA 81107, CZECHOSLOVAKIA
[3] UNIV UTRECHT, DEPT BIOCHEM MEMBRANES, CTR BIOMEMBRANES & LIPID ENZYMOL, 3584 CH UTRECHT, NETHERLANDS
来源：
FEBS LETTERS | 1993年 / 334卷 / 02期
关键词：
CHLOROPLAST PROTEIN IMPORT; TRANSIT PEPTIDE; CIRCULAR DICHROISM; PEPTIDE CONFORMATION; LIPID-PROTEIN INTERACTIONS;
D O I：
10.1016/0014-5793(93)81720-K
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Import of proteins into chloroplasts depends on an N-terminal transit sequence. Transit sequences contain little primary sequence similarity and therefore recognition of these sequences is thought to involve specific folding. To assess the conformational flexibility of the transit sequence, we studied the transit peptide of preferredoxin (trfd) by circular dichroism. In buffer, trfd is in a random coil conformation. A large increase in alpha-helix was induced in the presence of micelles or vesicles formed by anionic lipids. Less pronounced changes in secondary structure were induced by zwitterionic detergents but no changes were observed in the presence of neutral detergents or vesicles composed of phosphatidylcholine.
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页码：241 / 246
页数：6
相关论文
共 27 条
[1]  
CLINE K, 1985, J BIOL CHEM, V260, P3691
[2]   ENZYMATIC-SYNTHESIS OF PHOSPHATIDYLSERINE AND PURIFICATION BY CM-CELLULOSE COLUMN CHROMATOGRAPHY [J].
COMFURIUS, P ;
ZWAAL, RFA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 488 (01) :36-42
[3]   CHLOROPLAST PROTEIN TOPOGENESIS - IMPORT, SORTING AND ASSEMBLY [J].
DEBOER, AD ;
WEISBEEK, PJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1071 (03) :221-253
[4]   SYNTHESIS AND PROPERTIES OF ALKYLGLUCOSIDES WITH MILD DETERGENT ACTION - IMPROVED SYNTHESIS AND PURIFICATION OF BETA-1-OCTYL-GLUCOSE, BETA-1-NONYL-GLUCOSE AND BETA-1-DECYL-GLUCOSE - SYNTHESIS OF BETA-1-UNDECYLGLUCOSE AND BETA-1-DODECYLMALTOSE [J].
DEGRIP, WJ ;
BOVEEGEURTS, PHM .
CHEMISTRY AND PHYSICS OF LIPIDS, 1979, 23 (04) :321-335
[5]   THE CONFORMATIONAL-CHANGES OF APOCYTOCHROME C UPON BINDING TO PHOSPHOLIPID-VESICLES AND MICELLES OF PHOSPHOLIPID BASED DETERGENTS - A CIRCULAR-DICHROISM STUDY [J].
DEJONGH, HHJ ;
DEKRUIJFF, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1029 (01) :105-112
[6]   THE ROLE OF CHARGE AND HYDROPHOBICITY IN PEPTIDE LIPID INTERACTION - A COMPARATIVE-STUDY BASED ON TRYPTOPHAN FLUORESCENCE MEASUREMENTS COMBINED WITH THE USE OF AQUEOUS AND HYDROPHOBIC QUENCHERS [J].
DEKROON, AIP ;
SOEKARJO, MW ;
DEGIER, J ;
DEKRUIJFF, B .
BIOCHEMISTRY, 1990, 29 (36) :8229-8240
[7]  
Douce R, 1984, Subcell Biochem, V10, P1
[8]   THE CHLOROPLAST-TARGETING DOMAIN OF PLASTOCYANIN TRANSIT PEPTIDE CAN FORM A HELICAL STRUCTURE BUT DOES NOT HAVE A HIGH-AFFINITY FOR LIPID BILAYERS [J].
ENDO, T ;
KAWAMURA, K ;
NAKAI, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 207 (02) :671-675
[9]  
HANAHAN DJ, 1952, J BIOL CHEM, V195, P199
[10]   TRANSPORT AND ROUTING OF PROTEINS INTO CHLOROPLASTS [J].
KEEGSTRA, K .
CELL, 1989, 56 (02) :247-253
123