EXPRESSION OF CGRP MESSENGER-RNAS IN THE PINK SALMON, ONCORHYNCHUS-GORBUSCHA

被引：11
作者：
MAUBRAS, L
TABOULET, J
PIDOUX, E
LASMOLES, F
JULIENNE, A
MILHAUD, G
BENSON, AA
MOUKHTAR, MS
CRESSENT, M
机构：
[1] CHU ST ANTOINE, INSERM, U113, 27 RUE CHALIGNY, F-75571 PARIS 12, FRANCE
[2] CHU ST ANTOINE, CNRS, UA 163, F-75571 PARIS 12, FRANCE
[3] UNIV CALIF SAN DIEGO, SCRIPPS INST OCEANOG, LA JOLLA, CA 92093 USA
来源：
PEPTIDES | 1993年 / 14卷 / 05期
关键词：
PLASMA CGRP; CGRP RNAS; SALMON; REPRODUCTIVE CYCLE;
D O I：
10.1016/0196-9781(93)90074-Q
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the isolation of calcitonin gene-related peptide (CGRP) mRNAs and expression of these RNAs in different tissues in the pink salmon, Oncorhynchus gorbuscha. Hybridization of poly(A+) RNAs indicated a mature CGRP RNA of 1.1 kb. The CGRP-like immunoreactivity occurring in tissues and plasma had the same relative molecular weight as the synthetic molecule. Variations in CGRP plasma levels were observed during migration. spawning, and postspawning states. These data suggest that CGRP may play an important role during the reproductive cycle of salmon.
引用
收藏
页码：977 / 981
页数：5
相关论文
共 28 条
  • [1] ALTERNATIVE RNA PROCESSING IN CALCITONIN GENE-EXPRESSION GENERATES MESSENGER-RNAS ENCODING DIFFERENT POLYPEPTIDE PRODUCTS
    AMARA, SG
    JONAS, V
    ROSENFELD, MG
    ONG, ES
    EVANS, RM
    [J]. NATURE, 1982, 298 (5871) : 240 - 244
  • [2] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE
    AVIV, H
    LEDER, P
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (06) : 1408 - &
  • [3] CALCITONIN GENE-RELATED PEPTIDE IS A POTENT VASODILATOR
    BRAIN, SD
    WILLIAMS, TJ
    TIPPINS, JR
    MORRIS, HR
    MACINTYRE, I
    [J]. NATURE, 1985, 313 (5997) : 54 - 56
  • [4] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
  • [5] EXPRESSION OF THE HUMAN CALCITONIN CGRP GENE IN LUNG AND THYROID-CARCINOMA
    EDBROOKE, MR
    PARKER, D
    MCVEY, JH
    RILEY, JH
    SORENSON, GD
    PETTENGILL, OS
    CRAIG, RK
    [J]. EMBO JOURNAL, 1985, 4 (03) : 715 - 724
  • [6] STRUCTURAL CHARACTERIZATION OF CALCITONIN GENE-RELATED PEPTIDE PURIFIED FROM RABBIT INTESTINE
    EYSSELEIN, VE
    REEVE, JR
    STERNINI, C
    COMINELLI, F
    DAVIS, WM
    DAVIS, MT
    LEE, TD
    HO, FJ
    RIDOUT, D
    SHIVELY, JE
    [J]. PEPTIDES, 1991, 12 (02) : 289 - 295
  • [7] DISTRIBUTION OF CALCITONIN GENE-RELATED PEPTIDE AND CALCITONIN-LIKE IMMUNOREACTIVITY IN TROUT
    FOUCHEREAUPERON, M
    ARLOTBONNEMAINS, Y
    TABOULET, J
    MILHAUD, G
    MOUKHTAR, MS
    [J]. REGULATORY PEPTIDES, 1990, 27 (02) : 171 - 179
  • [8] GHATEI MA, 1984, REGUL PEPTIDES, V9, P330
  • [9] GIBSON SJ, 1984, J NEUROSCI, V4, P3101, DOI 10.1523/jneurosci.04-12-03101.1984
  • [10] IDENTIFICATION AND PARTIAL CHARACTERIZATION OF THE SALMON-CALCITONIN CGRP GENE BY POLYMERASE CHAIN-REACTION
    JANSZ, HS
    ZANDBERG, J
    [J]. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES, 1992, 657 : 63 - 69
123