KINETICS OF SINGLE AND COMPETITIVE PROTEIN ADSORPTION STUDIED BY REFLECTOMETRY AND STREAMING POTENTIAL MEASUREMENTS

被引：91

作者：

ELGERSMA, AV ^{[1
]}

ZSOM, RLJ ^{[1
]}

LYKLEMA, J ^{[1
]}

NORDE, W ^{[1
]}

机构：

[1] AKZO,CORP RES,6800 SB ARNHEM,NETHERLANDS

来源：

COLLOIDS AND SURFACES | 1992年 / 65卷 / 01期

关键词：

IMMUNOGAMMAGLOBULIN; PROTEIN ADSORPTION; REFLECTOMETRY; SERUM ALBUMIN; STREAMING POTENTIAL;

D O I：

10.1016/0166-6622(92)80171-W

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Changes in the reflectivity or streaming potential of polystyrene-covered silicon wafers or glass plates, respectively, upon the addition of proteins can be interpreted in terms of adsorbed amounts and/or adsorption rates. The rates of supply are given by hydrodynamic equations. We investigated the adsorption of BSA and four monoclonal immunoglobulins with different isoelectric points as a function of concentration and pH. Apart from the rate of supply, the amounts adsorbed also depend on the electrostatic interaction between proteins and surface. These observations complement adsorption measurements on polystyrene latices. The two techniques may also be used to obtain additional information regarding simultaneous and sequential adsorption of more than one protein.

引用

页码：17 / 28

页数：12

共 26 条

[1]

ALGERSMA AV, 1990, J COLLOID INTERF SCI, V138, P145

[2] SYNTHESIS OF A POLYSTYRENE LATEX WITH A POSITIVE, PH INDEPENDENT, SURFACE-CHARGE [J].