PURIFICATION OF A STAGE-SPECIFIC AND SEQUENCE-SPECIFIC DNA-BINDING PROTEIN FOR THE ARYLPHORIN GENE OF SARCOPHAGA-PEREGRINA

被引：5

作者：

KIM, JW ^{[1
]}

KOMANO, H ^{[1
]}

NATORI, S ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1089卷 / 01期

关键词：

ARYLPHORIN GENE; STAGE-SPECIFIC EXPRESSION; DNA-BINDING PROTEIN; (SARCOPHAGA-PEREGRINA);

D O I：

10.1016/0167-4781(91)90079-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein that binds specifically to the nucleotide sequence ACCACAACA located at residues -247 to -255 upstream of the +1 site of the arylphorin gene of Sarcophaga peregrina was purified to homogeneity from fat body nuclei of third instar larvae. This DNA-binding protein consisted of two subunits with molecular masses of 40 kDa and 42 kDa, respectively. Accurate transcription initiation of a truncated arylphorin gene in a nuclear extract of NIH-Sape-4 cells, an embryonic cell line of Sarcophaga, was significantly enhanced in the presence of the purified DNA-binding protein.

引用

页码：21 / 26

页数：6

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