SPECIFIC DETECTION OF CAMPYLOBACTER-CONCISUS BY PCR AMPLIFICATION OF 23S RDNA AREAS

被引：32

作者：

BASTYNS, K

CHAPELLE, S

VANDAMME, P

GOOSSENS, H

DEWACHTER, R

机构：

[1] UNIV INSTELLING ANTWERP,DEPT BIOCHEM,ONDERZOEKSGRP MOLEC BIOL,B-2610 ANTWERP,BELGIUM

[2] UNIV INSTELLING ANTWERP,DEPT GENEESKUNDE,MED MICROBIOL LAB,B-2610 ANTWERP,BELGIUM

[3] STATE UNIV GHENT,MICROBIOL MICROBIELE GENT LAB,B-9000 GHENT,BELGIUM

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 04期

关键词：

CAMPYLOBACTER CONCISUS; POLYMERASE CHAIN REACTION; 23S RDNA;

D O I：

10.1016/S0890-8508(95)90114-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The phenotypic detection of Campylobacter concisus, a species of considerable genomic and phenotypic heterogeneity, has proven to be rather tedious in the past. Although alternative methods like DNA:DNA hybridization, immunotyping or whole-cell protein electrophoresis are valuable for the specific detection of C. concisus, they are too laborious to be performed in routine settings. Hence a simple Campylobacter concisus-specific PCR assay was developed, based on a target sequence which comprises the most variable areas of 23S rDNA. The PCR assay was successfully evaluated on a broad selection of C. concisus strains and phylogenetically related bacteria. (C) 1995 Academic Press Limited

引用

页码：247 / 250

页数：4

共 14 条

[1] SPECIES-SPECIFIC DETECTION OF CAMPYLOBACTERS IMPORTANT IN VETERINARY-MEDICINE BY PCR AMPLIFICATION OF 23S RDNA AREAS [J].