DIFFERENTIATION-DEPENDENT EXPRESSION OF TENSIN AND CORTACTIN IN CHICKEN OSTEOCLASTS

被引:55
作者
HIURA, K
LIM, SS
LITTLE, SP
LIN, S
SATO, M
机构
[1] ELI LILLY & CO, LILLY CORP CTR, DEPT ENDOCRINE RES, LILLY RES LABS, INDIANAPOLIS, IN 46285 USA
[2] INDIANA UNIV, SCH MED, DEPT ANAT, INDIANAPOLIS, IN USA
[3] JOHNS HOPKINS UNIV, DEPT BIOPHYS, BALTIMORE, MD USA
来源
CELL MOTILITY AND THE CYTOSKELETON | 1995年 / 30卷 / 04期
关键词
TENSIN; CORTACTIN; VINCULIN; CHICKEN; OSTEOCLASTS; DIFFERENTIATION;
D O I
10.1002/cm.970300405
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The expression and localization of tensin and cortactin were examined in osteoclast precursors in comparison with isolated osteoclasts on various substrates. Initially, the ability of hen monocytes to differentiate into osteoclasts was evaluated on plastic or glass, and compared to differentiation on bone. Specifically, monocytes were isolated from the medullary bones of egg-laying hens maintained on a Ca-deficient diet. Differentiation was monitored morphologically and by quantitation of the ability to form Howship's lacunae in bone slices or resorb radiolabeled bone particles of 20-53 mu m diameter. These cells differentiated into tartrate resistant acid phosphatase (TRAP)-positive, bone resorbing, multinucleated syncytia in the presence of cytosine-1-beta-D-arabinofuranoside in a time dependent manner (day 1-6). Differentiation into osteoclast-like cells was similar whether cultured on plastic, on glass, or on bone. When compared to GAP-DH control levels, tensin and cortactin mRNA levels increased by 7- and 10-fold, respectively, by day 6. Tensin and cortactin protein levels also increased by 6- and 15-fold, respectively, by day 6. Immunofluorescence of differentiating precursors showed that tensin localized between regions of cell to cell contact and colocalized with vinculin in podosomes of osteoclast-like cells and of real osteoclasts. Cortactin immunofluorescence was not detectable in monocytes but localized inside tensin/vinculin podosome structures after fusion into osteoclast-like cells and in freshly isolated osteoclasts. Both tensin and cortactin were associated with attachment complexes used by osteoclast-like cells and osteoclasts to resorb bone. Specifically, punctate cortactin staining was observed inside tensin staining which formed a double ring structure at the membrane/bone interface of resorbing osteoclasts. These data showed that tensin and cortactin can be used as osteoclast differentiation markers, that participate in attachment complexes used to resorb bone, and that tensin may participate in the fusion process of osteoclast precursors. (C) 1995 Wiley-Liss, Inc.
引用
收藏
页码:272 / 284
页数:13
相关论文
共 50 条
[21]   Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes [J].
Yamamoto, Makiko ;
Taniguchi, Yukio ;
Kano, Koichiro ;
Yamada, Takahisa .
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2010, 9 (11) :1639-1645
[22]   Differentiation-dependent expression of heart type fatty acid-binding protein in C2C12 muscle cells [J].
Rump, R ;
Buhlmann, C ;
Borchers, T ;
Spener, F .
EUROPEAN JOURNAL OF CELL BIOLOGY, 1996, 69 (02) :135-142
[23]   Differentiation-dependent expression of interferon gamma and toll-like receptor 9 in 3T3-F442A adipocytes [J].
Khazen, Wael ;
M'Bika, Jean-Pierre ;
Collinet, Martine ;
Tramoni, Mathieu ;
Chany, Charles ;
Achour, Ammar ;
Forest, Claude .
BIOCHIMIE, 2007, 89 (05) :669-675
[24]   Differentiation-dependent photodynamic therapy regulated by porphobilinogen deaminase in B16 melanoma [J].
D Ickowicz Schwartz ;
Y Gozlan ;
L Greenbaum ;
T Babushkina ;
D J Katcoff ;
Z Malik .
British Journal of Cancer, 2004, 90 :1833-1841
[25]   N-cadherin function is required for differentiation-dependent cytoskeletal reorganization in lens cells in vitro [J].
Ferreira-Cornwell, MC ;
Veneziale, RW ;
Grunwald, GB ;
Menko, AS .
EXPERIMENTAL CELL RESEARCH, 2000, 256 (01) :237-247
[26]   Patterns of Crystallin Gene Expression in Differentiation State Specific Regions of the Embryonic Chicken Lens [J].
Ma, Zhiwei ;
Chauss, Daniel ;
Disatham, Joshua ;
Jiao, Xiaodong ;
Brennan, Lisa Ann ;
Menko, A. Sue ;
Kantorow, Marc ;
Hejtmancik, J. Fielding .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2022, 63 (04)
[27]   Differentiation-Dependent Regulation of Human Endogenous Retrovirus K Sequences and Neighboring Genes in Germ Cell Tumor Cells [J].
Mueller, Thomas ;
Hantsch, Claudia ;
Volkmer, Ines ;
Staege, Martin S. .
FRONTIERS IN MICROBIOLOGY, 2018, 9
[28]   Characterization and differentiation-dependent regulation of secreted phospholipases A2 in human keratinocytes and in healthy and psoriatic human skin [J].
Haas, U ;
Podda, M ;
Behne, M ;
Gurrieri, S ;
Alonso, A ;
Fürstenberger, G ;
Pfeilschifter, J ;
Lambeau, G ;
Gelb, MH ;
Kaszkin, M .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2005, 124 (01) :204-211
[29]   Gene expression profiles for in vitro human stem cell differentiation into osteoblasts and osteoclasts: a systematic review [J].
Ariffin, Shahrul Hisham Zainal ;
Lim, Ker Wei ;
Wahab, Rohaya Megat Abdul ;
Ariffin, Zaidah Zainal ;
Din, Rus Dina Rus ;
Shahidan, Muhammad Ashraf ;
Johari, Anis Nabilah ;
Abidin, Intan Zarina Zainol .
PEERJ, 2022, 10
[30]   Selenium accelerates chicken dendritic cells differentiation and affects selenoproteins expression [J].
Sun, Zhepeng ;
Liu, Ci ;
Pan, Tingru ;
Yao, Haidong ;
Li, Shu .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2017, 77 :30-37