SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF METHYL N-[5-[[4-(2-PYRIDINYL)-1-PIPERAZINYL]CARBONYL]-1H-BENZIMIDAZOL-2-YL] CARBAMATE IN RAT-BLOOD

被引：7

作者：

NAGARAJA, NV ^{[1
]}

SINGH, SK ^{[1
]}

GUPTA, RC ^{[1
]}

机构：

[1] CENT DRUG RES INST,DIV PHARMACOKINET & METAB,LUCKNOW 226001,UTTAR PRADESH,INDIA

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 664卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00475-K

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive high-performance liquid chromatographic assay has been developed and validated for the determination of methyl N-[5-[[4-(2-pyridinyl)-1-piperazinyl]carbonyl]-1H-benzimidazol-2yl] carbamate (CDRI compound 81/470) in normal rat blood. The method described herein is simple, with improved selectivity and sensitivity over a previously reported HPLC method. The limit of quantitation is 10 ng/ml (method 1) and 2.5 ng/ml (method 2) in blood, as compared with 40 ng/ml for the previous method. The standard curve in blood is linear over the concentration range 10-1000 ng/ml in method 1 and 2.5-1000 ng/ml in method 2 and the extraction recovery is higher than 80% for both methods.

引用

页码：472 / 477

页数：6

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