A LIQUID CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF CURCUMIN IN PGPR INOCULATED CURCUMA LONGA. L PLANT

A rapid and simple HPLC method has been developed for the quantification of curcumin in PGPR inoculated curcuma longa plant. Treatment of PGPR such as Pseudomonas fluorescens and Bacillus megaterium improved the content of curcumin in rhizomes sample. The PGPR treated rhizomes were higher in curcumin content than the PGPR untreated plant rhizomes sample. Analysis was performed using a C18 column (250 X 4.6 mm) by isocratic elution with 50mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40: 60) and detection at 420 nm and 340 nm using a photodiode array detector for curcumin respectively. The calibration plot was linear over the range studied (Curcumin: 100 - 3200ng/mL) with a correlation of 0.999. The method was also validated for the precision and recovery. Thus, the method is suitable for routine analysis of curcumin in PGPR inoculated curcuma longa plant sample 1, compared with PGPR uninoculated plant sample 2.