CONTENT OF TOTAL CHLOROPHYLL AND FREE AMINO ACIDS OF CHLORINA MUTANT OF ARABIDOPSIS THALIANA IN ARTIFICIAL GLUCOSE NUTRITION

When the lethal radiomutant chlorina 42 was cultivated on glucose containing and glucose-free medium the cotyledons of the mutant sown on glucose medium had a higher chlorophyll content than those of the mutant sown on glucose-free medium. In further cultivation of this mutant on glucose medium until flowering the total chlorophyll content is maintained at the same level, it increases slightly in the case of flowering plants. The mutant cultivated on glucose medium differs somewhat with regard to the content of free amino acids from the green control cultivated also on glucose medium over the whole vegetation period. The former has a higher glutamine content than the latter and in some growth phases also a higher content of asparagine. It is assumed that the insufficient photosynthetic activity is apparently not fully compensated by glucose and the plant has to supplement its energy balance by partial oxidative protein catabolism even under the conditions of artificial nutrition.