DETECTION OF CONFORMATIONAL MODIFICATIONS OF HEATED BETA-LACTOGLOBULIN BY IMMUNOCHEMICAL METHODS

被引：5

作者：

LALIGANT, A ^{[1
]}

MARTI, J ^{[1
]}

CHEFTEL, JC ^{[1
]}

DUMAY, E ^{[1
]}

CUQ, JL ^{[1
]}

机构：

[1] UNIV MONTPELLIER 2, GENIE BIOL & SCI ALIMENTS LAB, F-34095 MONTPELLIER 2, FRANCE

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1995年 / 43卷 / 11期

关键词：

BETA-LACTOGLOBULIN; THERMAL TREATMENT; CONFORMATIONAL MODIFICATION; IMMUNOCHEMICAL DETECTION;

D O I：

10.1021/jf00059a024

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

beta-Lactoglobulin (beta LG) was submitted to various thermal treatments (75 and 90 degrees C), and its conformational modifications were assessed by competitive radioimmunoassay (RIA) with rabbit antisera against native beta LG (beta LG(NAT)) or chemically modified beta LG obtained by performic oxidation (beta LG(OX)). Heat-processed beta LG reacted with both antisera. Molecule alteration was accompanied by an increased binding of polyclonal antibodies. Whatever the serum used, the RIA test revealed a thermal dependence that was influenced by pH (6.5 and 7.5). However, anti-beta LG(OX) antiserum was shown to be the most useful tool for monitoring slight denaturation structural changes after heating. The test accurately detected local modifications of beta LG epitopes that occurred after thermal processing of an industrial beta LG isolate.

引用

页码：2896 / 2903

页数：8

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