THE FREQUENCY OF THE MITOCHONDRIAL ALDEHYDE DEHYDROGENASE I-2 (ATYPICAL) ALLELE IN CAUCASIAN, ORIENTAL AND AFRICAN BLACK POPULATIONS DETERMINED BY THE RESTRICTION PROFILE OF PCR-AMPLIFIED DNA

The aldehyde dehydrogenase I (ALDH I) gene codes for a mitochondrial enzyme which plays a major role in hepatic alcohol detoxication. It has been related to alcohol flushing in Orientals bearing the atypical ALDH I-2 gene. The variant protein results from a lysine for glutamate substitution at position 487 (G --> A change in exon 12). A procedure for ALDH I-2 detection consisting in a differentiation between the 'atypical' allele and the 'wild' allele has been improved through PCR and subsequent MboII digestion. Blood samples collected on anticoagulant or directly absorbed on blotting paper were used for DNA amplification in the presence of two specific oligonucleotidic primers, each one able to incorporate a restriction site in the amplimer. After MboII digestion, PCR products were separated by polyacrylamide gel electrophoresis and then visualized with ethidium bromide. This technique permits a rapid and non-radioactive detection of atypical ALDH I-2 on a PCR product without the use of allele specific oligonucleotides. It was applied to the study of ALDH I-2 allele frequency in random population samples of three ethnic groups: Caucasians, Orientals and African blacks.