LOCALIZATION BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM MAPPING IN POTATO OF A MAJOR DOMINANT GENE CONFERRING RESISTANCE TO THE POTATO CYST NEMATODE GLOBODERA-ROSTOCHIENSIS

被引:129
作者
BARONE, A [1 ]
RITTER, E [1 ]
SCHACHTSCHABEL, U [1 ]
DEBENER, T [1 ]
SALAMINI, F [1 ]
GEBHARDT, C [1 ]
机构
[1] MAX PLANCK INST ZUCHTUNGSFORSCH,CARL VONLINNE WEG 10,W-5000 COLOGNE 30,GERMANY
来源
MOLECULAR & GENERAL GENETICS | 1990年 / 224卷 / 02期
关键词
Globodera rostochiensis; Potato; Resistance; Restriction fragment length polymorphism; Solanum spegazzinii;
D O I
10.1007/BF00271550
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A major dominant locus conferring resistance against several pathotypes of the root cyst nematode Globodera rostochiensis was mapped on the linkage map of potato using restriction fragment length polymorphism (RFLP) markers. The assessment of resistance versus susceptibility of the plants in the experimental population considered was based on an in vivo (pot) and an in vitro (petri dish) test. By linkage to nine RFLP markers the resistance locus Gro1 was assigned to the potato linkage group IX which is homologous to the tomato linkage group 7. Deviations from the additivity of recombination frequencies between Gro1 and its neighbouring markers in the pot test led to the detection of a few phenotypic misclassifications of small plants with poor root systems that limited the observation of cysts on susceptible roots. Pooled data from both tests provided better estimates of recombination frequencies in the linkage interval defined by the markers flanking the resistance locus. © 1990 Springer-Verlag.
引用
收藏
页码:177 / 182
页数:6
相关论文
共 20 条
[11]   THE LINKAGE OF MOLECULAR MARKERS TO A GENE CONTROLLING THE SYMPTOM RESPONSE IN MAIZE TO MAIZE-DWARF MOSAIC-VIRUS [J].
MCMULLEN, MD ;
LOUIE, R .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1989, 2 (06) :309-314
[12]  
MORTON NE, 1986, AM J HUM GENET, V38, P868
[13]   DEVELOPMENT OF AN EARLY TEST TO ASSESS RESISTANCE OF POTATOES TO GLOBODERA SP [J].
MUGNIERY, D ;
BALANDRAS, C .
POTATO RESEARCH, 1986, 29 (01) :45-53
[14]  
RITTER E, 1990, GENETICS, V125, P645
[15]  
ROSS H, 1962, BITT ZUCHTER, V32, P74
[16]  
ROSS H, 1986, Z PFLANZENZUCHTUNG S, V13
[17]   AN RFLP MARKER IN TOMATO LINKED TO THE FUSARIUM-OXYSPORUM RESISTANCE GENE I2 [J].
SARFATTI, M ;
KATAN, J ;
FLUHR, R ;
ZAMIR, D .
THEORETICAL AND APPLIED GENETICS, 1989, 78 (05) :755-759
[18]   RFLP MAPPING IN PLANT-BREEDING - NEW TOOLS FOR AN OLD SCIENCE [J].
TANKSLEY, SD ;
YOUNG, ND ;
PATERSON, AH ;
BONIERBALE, MW .
BIO-TECHNOLOGY, 1989, 7 (03) :257-264
[19]   PREPARATION OF MEGABASE-SIZED TOMATO DNA AND SEPARATION OF LARGE RESTRICTION FRAGMENTS BY FIELD INVERSION GEL-ELECTROPHORESIS (FIGE) [J].
VANDAELEN, RAJ ;
JONKERS, JJ ;
ZABEL, P .
PLANT MOLECULAR BIOLOGY, 1989, 12 (03) :341-352
[20]  
YOUNG ND, 1988, GENETICS, V120, P579